LC
(8) LC50 means that test substance concentration, calculated from experimentally-derived mortality data, that is lethal to 50 percent of a test population during continuous exposure over a specified period of time. (9) Loading means the ratio of fish biomass (grams, wet weight) to the volume (liters) of test solution in a test chamber or passing through it in a 24-hour period. (10) Static means the test solution is not renewed during the period of the test. (11) Test solution means the test substance and the dilution water in which the test substance is dissolved or suspended.