Technicon automated complement-fixation test.
Technicon automated complement-fixation test. A test to determine the brucellosis disease status of test-eligible cattle and bison when conducted according to instructions approved by APHIS and the State in which the test is to be conducted. Cattle and bison are classified according to the following reactions:
(i) Cattle and bison which are not official vaccinates:
(A) Fixation in a dilution of 1:10 or higher - brucellosis reactor;
(B) Fixation in a dilution of 1:5 but no fixation in a dilution of 1:10 - brucellosis suspect;
(C) No fixation in a dilution of 1:5 or lower - brucellosis negative;
(ii) Official vaccinates vaccinated with a Brucella abortus Strain 19 approved brucella vaccine:
(A) Fixation in a dilution of 1:20 or higher - brucellosis reactor;
(B) Fixation in a dilution of 1:10 but no fixation in a dilution of 1:20 - brucellosis suspect;
(C) Fixation in a dilution of 1:5 or less but no fixation in a dilution of 1:10 - brucellosis negative.
(iii) Official vaccinates vaccinated with an approved brucella vaccine other than a Brucella abortus Strain 19 approved brucella vaccine:
(A) Fixation in a dilution of 1:10 or higher - brucellosis reactor;
(B) Fixation in a dilution of 1:5 but no fixation in a dilution of 1:10 - brucellosis suspect;
(C) No fixation in a dilution of 1:5 or lower - brucellosis negative.