(a) A laboratory seeking certification for
microbiological analyses of water shall have available, or have access to, the
items required for the total coliform and fecal coliform procedures as listed
in the EPA "Manual for the Certification of Laboratories Analyzing Drinking
Water", Chapter 5, Section 3, Laboratory Equipment and Supplies which is hereby
incorporated by reference including any subsequent amendments and editions,
except that Sections 3.2.2, 3.5.4 and 3.11.6 are not incorporated by reference.
A copy is available for inspection at the Division of Public Health, 1915 Mail
Service Center, Raleigh, North Carolina 27699-1915. Nonprofit organizations or
government agencies may obtain a copy by contacting the EPA Drinking Water
Hotline at 800-426-4791. Other organizations may obtain a copy from the
National Technical Information Service at 800-336-4700 for thirty five dollars
In addition to the
items and procedures incorporated by reference in Paragraph (a) of this Rule
the laboratory shall have available the items and follow the procedures listed
in this Paragraph:
(1) Balance. Calibrate the
balance monthly using class S or S-1 reference weights. Check the balance with
a minimum of three traceable weights which bracket the laboratory weighing
(2) Autoclave. The autoclave
shall be checked at least weekly with a maximum registering thermometer. Heat
sensitive tape or spore strips or ampules may be used during each autoclave
cycle and results recorded.
Fecal Coliform Waterbath:
(A) A temperature
of 44.5C 0.2C shall be maintained.
(B) A thermometer graduated in 0.1C
increments shall be used to monitor temperature.
(C) The water level shall be sufficient to
reach the upper level of media in tubes.
(D) On days used, record temperature at least
twice per day with readings separated by at least four hours.