Minimum equipment requirements and methodology for microbiological analyses
shall be in accordance with the methods adopted in
which is hereby
incorporated by reference including any subsequent amendments and editions,
except that Nutrient Agar plus MUG in
incorporated by reference. A copy is available for inspection at the Division
of Public Health, 1915 Mail Service Center, Raleigh, North Carolina 27699-1915.
Copies of 40 CFR 141
may be obtained by contacting the EPA Drinking Water
Hotline at 800-426-4791 at no charge.
For total coliform analysis the
laboratory shall maintain certification for one or more of the approved methods
as specified in this Paragraph:
Membrane Filter Procedure (MF) may be used for drinking water when the sample
is free from interference (e.g. turbidity and particulates). A laboratory must
be approved for a second analytical procedure when MF is used.
(2) The Multiple Tube Fermentation (MTF)
procedure may be used for analyzing drinking water that contains particulates
or other interfering substances and may be used as the back up or the sole
(3) The ONPG-MUG
Test and the Colisure Test may be used for analyzing drinking water that
contains particulates. These methods may be used as the back up or the sole
laboratory shall maintain certification for one of the approved methods for
fecal coliform analysis.
all procedures in Paragraph (a) of this Rule the laboratory shall incubate
inoculated culture within 30 minutes of inoculation.