(A) Brucella canis tests.
(1) As used in this rule, a brucella canis
test means one of the following:
(a) A canine antibody test (card
test);
(b)
(a) An indirect fluorescent antibody test (IFA
test);
(c)
(b) A tube agglutination test (TAT test);
or
(d) A polymerase chain reaction test
(PCR test); or
(e)
(c) A brucella canis
culture test.
(2)
Except as provided below, the
The tests contained in paragraph (A)(1) of this
rule may only be conducted by a laboratory approved by or operated by the
department of agriculture
, except that a laboratory not
approved or operated by the Ohio department of agriculture may conduct an IFA
test.:
(a) A licensed-accredited
veterinarian or a registered veterinary technician shall conduct a card test
when testing for brucella canis; or
(b) A laboratory not approved or
operated by the Ohio department of agriculture may conduct an IFA
test.
(3)
Laboratories approved or operated by the Ohio department of agriculture may
combine no more than five samples for IFA testing. If the composite sample is
positive for brucella canis, each canine sample making up the composite sample
must be testing
tested individually.
(4) Any blood samples which are taken to
conduct a brucella canis test shall only be taken by a
an
licensed-accredited veterinarian, or by a
registered veterinary technician who is supervised by
a
an
licensed-accredited veterinarian.
(B) Brucella canis
classifications.
(1) Suspect.
(a) A canine shall be classified as brucella
canis
suspect, if it tests
positive for brucella canis using the following
tests:
(i) A rapid slide agglutination
test, otherwise known as step one of the card test;
(ii)
(i)
An IFA test conducted by a laboratory;
or
(iii) A PCR test; or
(iv)
(ii) A TAT test, at dilutions of 1/50 or
1/100.
(b) A canine test
which has been classified as brucella canis
suspect according to
the paragraph
above
(B)(1)(a), shall
either:
(i) Be referred to the Ohio department
of agriculture for confirmation of its brucella canis status; or
(ii) Be humanely euthanized.
(2) Negative.
A canine shall be classified as brucella canis negative if,
through tests conducted by the Ohio department of agriculture or an approved
laboratory, the canine tests negative for brucella canis using the following
tests:
(a) A card test;
(b)
(a)
An IFA test; or
(c)
(b) A TAT test
(3) Positive.
A canine shall be classified as brucella canis positive if,
through tests conducted by the Ohio department of agriculture or an approved
laboratory, the canine tests positive for brucella canis using the following
tests:
(a) A card test;
(b) An IFA test;
(c)
(a) A
TAT test, at dilutions 1/200; or
(d)
(b) A brucella canis
culture test.
(4)
Reclassification.
Brucella canis positive animals may be reclassified by the Ohio
department of agriculture to brucella canis suspect pending the
following:
(a) The animal's
history;
(b) Clinical signs of the
animal;
(c) Vaccination
history;
(d) An epidemiological
study of the animal;
(e)
Examination; and
(f) Further
testing.
(C)
Identification and reporting.
(1) All
accredited
-licensed veterinarians who
conduct a brucella canis test, submits a sample for brucella canis testing, or
employs any other method of detection for brucella canis, shall include in the
report as required in division (C) of section
941.24 of the Revised Code, on a
form approved by the Ohio department of agriculture, the following information:
(a) Name and address of the canine's
owner;
(b) Date
and
of test
or sample collection;
(c)
Identification, age, sex, and breed of each individual animal tested or
sampled; and,
(d) The results of
the test performed, if available.
(2) All accredited-licensed veterinarians and laboratories who
conduct brucella canis testing shall report the results of the tests to the
Ohio department of agriculture within seven days on a form approved by the Ohio
department of agriculture.
(3) An
accredited
-licensed veterinarian must
identify any canine to be tested for brucella canis with any of the following
items which have been approved by the Ohio department of agriculture:
(a) A microchip;
(b) A tattoo;
(c) A tamper proof eartag; or
(d) A neck chain with an individual official
USDA identification tag may only be used by USDA licensed kennels.
(D) Quarantine.
(1)
The
Pursuant to Chapter 941 of the Ohio Revised Code,
the Ohio department of agriculture shall quarantine any canine on any
premises that has been exposed to or diagnosed as
brucella canis suspect or brucella canis positive animal.
(2) A brucella canis suspect canine shall be
released from quarantine upon a
two negative test
tests at least sixty days apart that were
conducted by the Ohio department of agriculture or an approved
laboratory.
(3) A brucella canis
positive canine on an Ohio department of agriculture licensed kennel premises
shall be released from quarantine upon either:
(a) The animal has been humanely euthanized;
or
(b) All of the following have
been met:
(i) Ovario-hysterectomy or
castration;
(ii) The owner has been
advised as to the zoonotic potential; and
(iii) The canine cannot be offered for sale,
trade, barter, or otherwise cannot leave the premises without prior permission
of the Ohio department of agriculture.
(4) A brucella canis
positive canine not on
an Ohio department of agriculture licensed kennel premises shall be released
from quarantine upon the following conditions being met:
(a) Ovario-hysterectomy or
castration;
(b) An epidemiological
study conducted by the Ohio department of agriculture;
(c)
A
Two negative test
tests at least sixty
days apart that were conducted by the Ohio department of agriculture or
an approved laboratory;
(d) The
owner has been advised as to the zoonotic potential; and,
(e) The canine cannot be offered for sale,
trade, barter, or otherwise cannot leave the premises without prior permission
of the Ohio department of agriculture.
(E) Selling, movement, and disposal of
animals.
(1) No canine known to be brucella
canis suspect or brucella canis positive shall be offered for sale, trade,
barter, or otherwise removed from the premises.
(2) Puppies born to a brucella canis
suspect
dams
dam
shall not be offered for sale, trade, barter, or be removed from the premises
unless the dam is later classified as brucella canis
negative.
These puppies may be isolated and quarantined in
accordance with paragraph (D) of rule 901:1-5-12 of the Administrative Code and
shall be tested after they reach six months of age and must be re-tested no
sooner than sixty days after the initial test.
(3) Puppies born to a brucella
positive dam
must not be offered for sale, barter, or otherwise removed from the premises
without prior permission of the department. These puppies may be isolated and
quarantined in accordance with
paragraph (D) of
rule
901:1-5-12 of the Administrative Code and shall be tested after six months
of age
and must be re-tested no sooner than sixty days
after the initial test.
(4)
Puppies under six-months of age which are classified as brucella canis positive
may be retested after six-months of age and must be
re-tested no sooner than sixty days after the initial test.
Notes
Ohio Admin. Code 901:1-5-12
Effective:
11/19/2018
Five Year Review (FYR) Dates:
9/4/2018 and
11/19/2023
Promulgated
Under: 119.03
Statutory
Authority: 941.02,
941.03,
941.22
Rule
Amplifies: 941.02
Prior
Effective Dates: 08/22/2015