(A) Brucella canis tests.
(1) As used in this rule, a
" brucella canis test
" means one of the following:
(a) An indirect fluorescent antibody test (IFA test);
(b) A tube agglutination test (TAT test); or
(c) A brucella canis culture test.
(2)
"Accredited laboratory" means a laboratory which maintains current accreditation through the international organization for standardization (ISO) or the American association of veterinary laboratory diagnosticians (AAVLD).
(2)(3) The tests contained in paragraph (A)(1)
paragraphs (A)(1)(a) to (A)(1)(c) of this rule may only be conducted by a
an accredited laboratory approved by or operated by the Ohio department of agriculture
agriculture's diagnostic laboratory, except that a laboratory not approved or operated by the Ohio department of agriculture may conduct an IFA test.
(3)(4)
Laboratories approved
Accredited laboratories or operated by the Ohio department of agriculture
agriculture's diagnostic laboratory may combine no more than five samples for IFA testing. If the composite sample is positive for brucella canis, each canine sample making up the composite sample must be tested individually.
(4)(5) Any blood samples which are taken to conduct a brucella canis test shall only be taken by an accredited veterinarian, or by a registered veterinary technician who is supervised by an accredited veterinarian.
(6)
A validated testing methodology approved and administered by the Ohio department of agriculture's diagnostic laboratory.
(B) Brucella canis classifications.
(1) Suspect.
(a) A canine shall be classified as brucella canis
suspect, if
it
the canine tests
positive for brucella canis using the following tests:
(i) An IFA test conducted by a laboratory; or
(ii) A TAT test, at dilutions of 1/50
1:50 or 1/100
1:100.
(b) A canine
test which has been classified as brucella canis
suspect according to paragraph
(B)(1)(a)
(B)(1)(a)(i) or (B)(1)(a)(ii) of this rule, shall either:
(i) Be referred to the Ohio department of agriculture for confirmation of its
the canine's brucella canis status; or
(ii) Be humanely euthanized.
(2) Negative.
A canine shall be classified as brucella canis negative if, through tests conducted by the Ohio department of agriculture
agriculture's diagnostic laboratory or an approved
accredited laboratory, the canine tests negative for brucella canis using the following tests:
(a) An IFA test; or
(b) A TAT test.
(3) Positive.
A canine shall be classified as brucella canis positive if, through tests conducted by the Ohio department of agriculture or an approved
accredited laboratory, the canine tests positive for brucella canis using the following tests:
(a) A TAT test, at dilutions 1/200
1:200; or
(b) A brucella canis culture test.
(4) Reclassification.
Brucella canis positive animals may be reclassified by the Ohio department of agriculture to brucella canis suspect pending the following:
(a) The animal's history;
(b) Clinical signs of the animal;
(c) Vaccination and treatment history;
(d) An epidemiological study of the animal;
(e) Examination; and
(f) Further testing at the discretion of the Ohio department of agriculture.
(C) Identification and reporting.
(1) All accredited veterinarians who conduct a brucella canis test,
submits
submit a sample for brucella canis testing, or
employs
employ any other method of detection for brucella canis, shall include in the report
toas required in division (C) of section 941.24 of the Revised Code, on a form approved by the Ohio department of agriculture, the following information:
(a) Name
, mailing address and
residence address of the canine's owner;
The residence address of the animal if different from the canine's owner;
(b) Date of test or sample collection;
(c)
Identification
Official identification as outlined in paragraph (C)(4) of this rule, age, sex, and breed of each individual animal tested or sampled; and,
(d) The results of the test performed, if available.
(2) All accredited veterinarians and laboratories who conduct brucella canis testing or submit samples for brucella canis testing at a laboratory other than the Ohio department of agriculture's diagnostic laboratory shall report the results of the tests to the Ohio department of agriculture within seven days on a form approved by the Ohio department of agriculture. The report shall include all information contained in paragraphs (C)(1)(a) to (C)(1)(d) of this rule and be submitted electronically to the state veterinarian's office.
(3)
All accredited veterinarians who draw blood for brucella canis testing shall submit the blood sample to an accredited laboratory within seven days.
(3)(4) An accredited veterinarian must identify any canine to be tested for brucella canis with any of the following items which
have been
are approved by the Ohio department of agriculture:
(a) A microchip;
(b)
A collar affixed around the dog's neck with a tag made of metal or aluminum that contains the following engraved, stamped, or imprinted information:
(i)
The individual dog's number; and
(ii)
The Ohio high volume breeder license number.
(b) A tattoo;
(c) A tamper proof eartag; or
(d) A neck chain with an individual official USDA identification tag may only be used by USDA licensed kennels.
(D) Quarantine.
(1) Pursuant to Chapter 941. of the Revised Code, the Ohio department of agriculture shall quarantine any canine on any premises that has been exposed to or diagnosed as brucella canis suspect or brucella canis positive animal.
(2)
A whole kennel test is defined as all test-eligible dogs located at a premises being tested within thirty days. Test-eligible dogs are dogs that are six months of age and older.
(2)(3) A brucella canis
positive, exposed, or suspect canine shall be released from quarantine upon
:
two negative tests at least sixty days apart that were conducted by the Ohio department of agriculture or an approved laboratory.
(a)
An epidemiological study conducted by the Ohio department of agriculture;
(b)
The owner has been advised by the Ohio department of agriculture as to the zoonotic potential; and
(c)
Two negative whole kennel IFA or TAT tests at least sixty days apart that were conducted by the Ohio department of agriculture or the national veterinary services laboratory; or
(d)
The canine has been humanely euthanized or is otherwise deceased and documented by a licensed veterinarian or confirmed by the Ohio department of agriculture.
(4)
Once a canine or a premises is under a quarantine order for brucella canis, all testing must be conducted by the Ohio department of agriculture's diagnostic laboratory or the national veterinary services laboratory in order for the quarantine order to be modified or lifted.
(3) A brucella canis positive canine on an Ohio department of agriculture licensed kennel premises shall be released from quarantine upon either:
(a) The animal has been humanely euthanized; or
(b) All of the following have been met:
(i) Ovario-hysterectomy or castration;
(ii) The owner has been advised as to the zoonotic potential; and
(iii) The canine cannot be offered for sale, trade, barter, or otherwise cannot leave the premises without prior permission of the Ohio department of agriculture.
(4) A brucella canis positive canine not on an Ohio department of agriculture licensed kennel premises shall be released from quarantine upon the following conditions being met:
(a) Ovario-hysterectomy or castration;
(b) An epidemiological study conducted by the Ohio department of agriculture;
(c) Two negative tests at least sixty days apart that were conducted by the Ohio department of agriculture or an approved laboratory;
(d) The owner has been advised as to the zoonotic potential; and,
(e) The canine cannot be offered for sale, trade, barter, or otherwise cannot leave the premises without prior permission of the Ohio department of agriculture.
(E) Selling, movement, and disposal of animals.
(1) No canine known to be brucella canis exposed,brucella canis suspect
, or brucella canis positive shall be offered for sale, trade, barter, or otherwise removed from the premises without prior written permission from the Ohio department of agriculture.
(2) Puppies born to a brucella canis
suspect or positive
dam shall not be offered for sale, trade, barter, or be removed from the premises unless the dam is later classified as brucella canis
negative by the Ohio department of agriculture or the national veterinary services laboratory. These puppies may be isolated and quarantined in accordance with paragraph (D) of rule
901:1-5-12 of the Administrative Code and shall be tested after
they reach six months of age and must be re-tested no sooner than sixty days after the initial test
in accordance with paragraphs (A)(1)(a) to (A)(1)(c) of this rule.
(3) Puppies born to a brucella positive dam must not be offered for sale, barter, or otherwise removed from the premises without prior permission of the department. These puppies may be isolated and quarantined in accordance with paragraph (D) of rule 901:1-5-12 of the Administrative Code and shall be tested after six months of age and must be re-tested no sooner than sixty days after the initial test.
(4)(3) Puppies under six-months of age which are classified as brucella canis
suspect or positive may be retested
re-tested after six-months of age and must be re-tested no sooner than sixty days after the initial test in accordance with paragraphs (A)(1)(a) to (A)(1)(c) of this rule.
Notes
Ohio Admin. Code 901:1-5-12
Effective: 4/1/2025
Five Year Review (FYR) Dates: 1/14/2025 and 04/01/2030
Promulgated Under: 119.03
Statutory Authority: 941.02, 941.03, 941.22
Rule Amplifies: 941.02
Prior Effective Dates: 08/22/2015, 11/19/2018
