Tenn. Comp. R. & Regs. 0400-45-01-.14 - LABORATORY CERTIFICATION
Chemistry
Fee Type |
Fee in Dollars |
1. Administrative In-State |
$1000 |
2. Administrative Out-of-State |
$750 |
3. General Chemistry-Turbidity, Corrosivity, pH |
$500 |
4. Inorganics - Trace Metals, Sodium, Nitrite, Nitrate, Fluoride, Sulfate, Cyanide, Asbestos, Chlorite, and Bromate |
$500 |
5. Organics |
$500 |
6. Disinfection Byproducts - Trihalomethanes and Haloacetic acids |
$500 |
7. Polychlorinated Biphenyls (PCB) |
$500 |
8. Radiochemistry |
$500 |
9. Enzyme Substrate - Total Coliform and E.-Coli |
$500 |
10. Membrane Filter - Total Coliform, Fecal Coliform, E.-Coli |
$500 |
11. Heterotrophic Plate Count |
$500 |
12. Enterococci |
$500 |
The following table summarizes the 80% Rule.
Analyte(s) |
PE Success Requirement |
Vinyl Chloride |
100% |
20 VOCs |
80% * |
4 Regulated THMs |
100% |
5 Regulated HAA5s |
100% |
*A lab will not maintain certification for analyte(s) which it repeatedly fails.
TABLE 0400-45-01-.14(10)(a) 1.
Organism |
Methodology |
Citation1 |
Total Coliforms2 |
Total Coliform Fermentation Technique3,4,5 |
9221 A, B |
Total Coliform Membrane Filter Technique6 |
9222 A, B, C |
|
Presence-Absence (P-A) Coliform Test5,7 |
9221 |
|
ONPG-MUG Test8 Colisure Test9 E*Colite®Test10 m-ColiBlue24®Test11 Readycult®Coliforms 100 Presence/Absence Test12 Membrane Filter Technique using Chromocult®Coliform Agar13 Colitag®Test14 |
9223 |
Footnotes
1 Methods 9221 A, B; 9222 A, B, C; 9221 D and 9223 are contained in Standard Methods for the Examination of Water and Wastewater, 18th edition (1992) and 19th edition (1995) American Public Health Association, 1015 Fifteenth Street NW, Washington, D.C. 20005; either edition may be used. In addition, the following online versions may also be used: 9221 A, B, D-99, 9222 A, B, C-97 and 9223 B-97. Standard Methods Online are available at http://www.standardmethods.org. The year in which each method was approved by the Standard Methods Committee is designated by the last two digits in the method number. The methods listed are the only online versions that may be used.
2 The time from sample collection to initiation of analysis may not exceed 30 hours. Systems are encouraged but not required to hold samples below 10°C during transit.
3 Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-negative rate for total coliform, using lactose broth, is less than 10 percent.
4 If inverted tubes are used to detect gas production, the media should cover these tubes at least one-half to two-thirds after the sample is added.
5 No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
6 MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total coliform and Escherichia coli in water'' by Brenner, K.P., et al., 1993, Appl. Environ. Microbiol. 59:3534-3544. Also available from the Office of Water Resource Center (RC-4100), 401 M. Street SW, Washington, DC 20460, EPA/600/J-99/225.
7 Six-times formulation strength may be used if the medium is filter-sterilized rather than autoclaved.
8 The ONPG-MUG Test is also known as the Autoanalysis Colilert System.
9 A description of the Colisure Test, Feb 28, 1994, may be obtained from IDEXX Laboratories, Inc., One IDEXX Drive, Westbrook, Maine 04092. The Colisure Test may be read after an incubation time of 24 hours.
10 A description of the E*Colite® Test, "Presence/Absence for Coliforms and E. Coli in Water,'' Dec 21, 1997, is available from Charm Sciences, Inc., 36 Franklin Street, Malden, MA 02148-4120.
11 A description of the m-ColiBlue24® Test, Aug 17, 1999, is available from the Hach Company, 100 Dayton Avenue, Ames, IA 50010.
12 The Readycult® Coliforms 100 Presence/Absence Test is described in the document, "Readycult® Coliforms 100 Presence/Absence Test for the Detection and Identification of Coliform Bacteria and Escherichla coli in finished water", November 2000, Version 1.0, available from EM Science (an affiliate of Merck KggA, Darmstadt Germany), 480 S. Democrat Road, Gibbstown, NJ 08027-1297. Telephone number is (800) 222-0342, e-mail address is: adellenbusch@emscience.com.
13 Membrane Filter Technique using Chromocult® Coliform Agar is described in the document, "Chromocult® Coliform Presence/Absence Membrane Filter Test Method for Detection and Identification of Coliform Bacteria and Escherichla coli in finished water", November 2000, Version 1.0, available from EM Science (an affiliate of Merck KggA, Darmstadt Germany), 480 S. Democrat Road, Gibbstown, NJ 08027-1297. Telephone number is (800) 222-0342, e-mail address is: adellenbusch@emscience.com.
14 Colitag® product for the determination of the presence/absence of total coliforms and E. coli is described in "Colitag® Product as a Test for Detection and Identification of Coliforms and E. coli Bacteria in Drinking Water and Source Water as required in National Primary Drinking Water Regulations," August 2001, available from CPI International, Inc., 5580 Skylane Blvd., Santa Rosa, CA, 95403 telephone (800) 878-7654, Fax (707) 545-7901, Internet address http://www.cpiinternational.com.
TABLE 0400-45-01-.14(10)(a) 1.(i)
Organism |
Methodology |
SM 21st Edition1 |
Other |
Total Coliforms |
Total Coliform Fermentation Technique |
9221 A, B |
|
Total Coliform Membrane Filter Technique |
9222 A, B, C |
||
Presence-Absence (PA) Coliform Test |
9221 D |
||
ONPG-MUG Test |
9223 |
||
ColitagTM |
Modified ColitagTM2 |
Footnotes
1 Standard Methods for the Examination of Water and Wastewater, 21st edition (2005). Available from American Public Health Association, 800 I Street NW, Washington, DC 20001- 3710
2 Modified ColitagTM Method. "Modified ColitagTM Test Method for the Simultaneous Detection of E. Coli and other Total Coliforms in Water (ATP D05-0035)," August 28, 2009. Available at http://www.nemi.gov or from CPI International, 5580 Skylane Boulevard, Santa Rosa, CA 95403.
TABLE 0400-45-01-.14(10)(a) 3.(xi)
Alternative Testing Methods for E. coli |
|||||
Organism |
Methodology |
SM 20thEdition1 |
SM 21stEdition2 |
SM Online3 |
Other |
E. coli |
ONPG-MUG Test |
9223 B |
|||
Modified ColitagTM4 |
Footnotes
1 Standard Methods for the Examination of Water and Wastewater, 20th Edition (1998). Available from American Public Health Association, 800 I Street NW, Washington, DC 20001-3710.
2 Standard Methods for the Examination of Water and Wastewater, 21st edition (2005). Available from American Public Health Association, 800 I Street, NW, Washington, DC 20001- 3710
3 Standard Methods Online are available at http://www.standardmethods.org. The year in which each method was approved by the Standard Methods Committee is designated by the last two digits in the method number. The methods listed are the only online versions that may be used.
4 Modified ColitagTM Method. "Modified ColitagTM Test Method for the Simultaneous Detection of E. Coli and other Total Coliforms in Water (ATP D05-0035)," August 28, 2009. Available at http://www.nemi.gov or from CPI International, 5580 Skylane Boulevard, Santa Rosa, CA 95403.
TABLE 0400-45-01-.14(10)(a) 4.
Organism |
Methodology |
Citation1 |
Total Coliform2 |
Total Coliform Fermentation Technique3,4,5 Total Coliform Membrane Filter Technique6 ONPG-MUG Test7 |
9221 A, B, C 9222 A, B, C 9223 |
Fecal Coliforms2 |
Fecal Coliform Procedure8 Fecal Coliform Filter Procedure |
9221 E 9222 D |
Heterotrophic bacteria2 |
Pour Plate MethodSimPlate9 |
9215 B |
Footnotes
1. Except where noted, all methods refer to Standard Methods for the Examination of Water and Wastewater, 18th edition (1992), 19th edition (1995), or 20th edition (1998), American Public Health Association, 1015 Fifteenth Street NW., Washington, DC 20005. The cited methods published in any of these three editions may be used. In addition, the following online versions may also be used: 2130 B-01, 9215 B-00, 9221 A, B, C, E-99, 9222 A, B, C, D-97, and 9223 B-97. Standard Methods Online are available http://www.standardmethods.org. The year in which each method was approved by the Standard Methods Committee is designated by the last two digits in the method number. The methods listed are the only Online versions that may be used.
2. The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10 deg. C during transit. {8 hour holding time only applies to parts (2)(a)1. and (5)(b)2. of Rule 0400-45-01-.31 }
3. Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-negative rate for total coliform, using lactose broth, is less than 10 percent.
4. Media should cover inverted tubes at least one-half to two-thirds after the sample is added.
5. No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
6. MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total coliform and Escherichia coli in water'' by Brenner, K.P., et. al., 1993, Appl. Environ. Microbiol. 59:3534-3544. Also available from the Office of Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW., Washington DC 20460, EPA/600/J-99/225. Verification of colonies is not required.
7. The ONPG-MUG Test is also known as the Autoanalysis Colilert System.
8. A-1 Broth may be held up to seven days in a tightly closed screw cap tube at 4 deg. C.
9. A description of the SimPlate method, "IDEXX SimPlate TM HPC Test Method for Heterotrophs in Water'', November 2000, can be obtained from IDEXX Laboratories, Inc., One IDEXX Drive, Westbrook, Maine 04092, telephone (800) 321-0207.
Organism |
Methodology |
SM 21st Edition1 |
Total Coliform |
Total Coliform Fermentation Technique |
9221 A, B, C |
Total Coliform Membrane Filter Technique |
9222 A, B, C |
|
ONPG-MUG Test |
9223 |
|
Fecal Coliforms |
Fecal Coliform Procedure |
9221 E |
Fecal Coliform Filter Procedure |
9222 D |
|
Heterotrophic Bacteria |
Pour Plate Method |
9215 B |
Footnotes
1 Standard Methods for the Examination of Water and Wastewater, 21st edition (2005). Available from American Public Health Association, 800 I Street, NW, Washington, DC 20001-3710.
TABLE 0400-45-01-.14(10)(a) 5.
Parameter and units |
Method1 |
EPA |
Standard methods 18th,19th, 20th Ed. |
Standard methods online |
AOAC, ASTM, USGS |
Other |
E. coli , number per 100 ml16 |
MPN2,3,4multiple tube, |
9221B / 9221F6,8 |
9221B-99 / 9221F6,8 |
|||
multiple tube/multiple well |
9223B7 |
9223B-977 |
991.155 |
Colilert®7,10 Colilert-18®7,9,10 |
||
MF two step |
1103.113 |
9222B/9222G12, 9213D |
9222B-97 / 9222G12 |
D5392-934 |
||
MF single step |
160314 , 160415 |
mColiBlue-24®11 |
Footnotes
1. The method must be specified when results are reported.
2. Tests must be conducted to provide organism enumeration (density). Select the appropriate configuration of tubes/filtrations and dilutions/volumes to account for the quality, character, consistency, and anticipated organism density of the water sample.
3. To assess the comparability of results obtained with individual methods, it is suggested that side-by-side tests be conducted across seasons of the year with the water samples routinely tested in accordance with the most current Standard Methods for the Examination of Water and Wastewater or EPA alternate test procedure (ATP) guidelines.
4. ASTM. 2000, 1999, 1996. Annual Book of ASTM Standards-Water and Environmental Technology. Section 11.02. ASTM International. 100 Barr Harbor Drive, West Conshohocken, PA 19428.
5. AOAC. 1995. Official Methods of Analysis of AOAC International, 16th Edition, Volume I, Chapter 17. Association of Official Analytical Chemists International. 481 North Frederick Avenue, Suite 500, Gaithersburg, MD 20877-2417.
6. The multiple-tube fermentation test is used in 9221B.1. Lactose broth may be used in lieu of lauryl tryptose broth (LTB), if at least 25 parallel tests are conducted between this broth and LTB using the water samples normally tested, and this comparison demonstrates that the false-positive rate and false-negative rate for total coliform using lactose broth is less than 10 percent. No requirement exists to run the completed phase on 10 percent of all total coliform-positive tubes on a seasonal basis.
7. These tests are collectively known as defined enzyme substrate tests, where, for example, a substrate is used to detect the enzyme b-glucuronidase produced by E. coli.
8. After prior enrichment in a presumptive medium for total coliform using 9221B.1, all presumptive tubes or bottles showing any amount of gas, growth or acidity within 48 h ± 3 h of incubation shall be submitted to 9221 F. Commercially available EC-MUG media or EC media supplemented in the laboratory with 50 ±g/mL of MUG may be used.
9. Descriptions of the Colilert®, Colilert-18®, Quanti-Tray®, and Quanti-Tray®/2000 may be obtained from IDEXX Laboratories, Inc., 1 IDEXX Drive, Westbrook, ME 04092.
10. Descriptions of the Colilertn, Colilert-18®, Quanti-Tray®, and Quanti-Tray®/2000 may be obtained from IDEXX Laboratories, Inc., 1 IDEXX Drive, Westbrook, ME 04092.
11. A description of the mColiBlue24® test, Total Coliforms and E. coli, is available from Hach Company, 100 Dayton Ave., Ames, IA 50010.
12. Subject total coliform positive samples determined by 9222B or other membrane filter procedure to 9222G using NA MUG media.
13. USEPA. 2002. Method 1103.1: Escherichia coli (E. coli) In Water By Membrane Filtration Using membrane-Thermotolerant Escherichia coli Agar (mTEC). U.S. Environmental Protection Agency, Office of Water, Washington, DC, EPA-821-R-02-020.
14. USEPA. 2002. Method 1603: Escherichia coli (E. coli) In Water By Membrane Filtration Using Modified membrane-Thermotolerant Escherichia coli Agar ( modified mTEC). U.S. Environmental Protection Agency, Office of Water, Washington, DC, EPA-821-R-02-023.
15. Preparation and use of MI agar with a standard membrane filter procedure is set forth in the article, Brenner et al. 1993. "New Medium for the Simultaneous Detection of Total Coliform and Escherichia coli in Water.'' Appl. Environ. Microbiol. 59:3534-3544 and in USEPA. 2002. Method 1604: Total Coliforms and Escherichia coli (E. coli) in Water by Membrane Filtration by Using a Simultaneous Detection Technique (MI Medium).U.S. Environmental Protection Agency, Office of Water, Washington, DC, EPA 821-R-02-024.
16. Recommended for enumeration of target organism in ambient water only.
Analytical Methods for Source Water Monitoring |
||
Fecal Indicator1 |
Methodology |
Method citation |
E. coli |
Colilert3 |
9223 B.2 |
Colisure3 |
9223 B.2 |
|
Membrane Filter Method with MI Agar |
EPA Method 1604.4 |
|
m-ColiBlue24 Test5 |
||
E*Colite Test6 |
||
EC-MUG7 |
||
NA-MUG7 |
9221 F.2 |
|
9222 G.2 |
||
Enterococci |
Multiple-Tube Technique |
9230B.2 |
Membrane Filter Technique |
9230C.2 |
|
Membrane Filter Technique |
EPA Method 1600.8 |
|
Enterolert9 |
Analyses must be conducted in accordance with the documents listed below. The Director of the Federal Register approves the incorporation by reference of the documents listed in footnotes 2-11 in accordance with 5 U.S.C. 552(a) and 1 CFR part 51. Copies of the documents may be obtained from the sources listed below. Copies may be inspected at EPA's Drinking Water Docket, EPA West, 1301 Constitution Avenue, NW., EPA West, Room B102, Washington DC 20460 (Telephone: 202-566-2426); or at the National Archives and Records Administration (NARA). For information on the availability of this material at NARA, call 202-741-6030, or go to:
http://www.archives.gov/federal_register/code_of_federal_regulations/ibr_locations.html.
Footnotes
1 The time from sample collection to initiation of analysis may not exceed 30 hours. The ground water system is encouraged but not required to hold samples below 10° C during transit.
2 Methods are described in Standard Methods for the Examination of Water and Wastewater 20th edition (1998) and copies may be obtained from the American Public Health Association, 1015 Fifteenth Street NW., Washington DC 20005-2605.
3 Medium is available through IDEXX Laboratories, Inc., One IDEXX Drive, West Brook, Maine 04092.
4 EPA Method 1604: Total Coliforms and Escherichia coli in Water by Membrane Filtration Using a Simultaneous Detection Technique (MI Medium); September 2002, EPA 821-R-02-024. Method is available at http://www.epa.gov/nerlccwww/1604sp02.pdf or from EPA's Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW., Washington DC 20460.
5 A description of the m-ColiBlue24 Test, "Total Coliforms and E. coli Membrane Filtration Method with m-ColiBlue24 Broth," Method No. 10029 Revision 2, August 17, 1999, is available from Hach Company, 100 Dayton Ave., Ames IA 50010 or from EPA's Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW., Washington DC 20460.
6 A description of the E*Colite Test, "Charm E*Colite Presence/Absence Test for Detection and Identification of Coliform Bacteria and Escherichia coli in Drinking Water, January 9, 1998, is available from Charm Sciences, Inc., 659 Andover St., Lawrence, MA 01843-1032 or from EPA's Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW., Washington DC 20460.
7 EC-MUG (Method 9221F) or NA-MUG (Method 9222G) can be used for E. coli testing step as described in 141.21(f)(6)(i) or (ii) after use of Standard Methods 9221 B, 9221 D, 9222 B, or 9222 C.
8 EPA Method 1600: Enterococci in Water by Membrane Filtration Using membrane-Enterococcus Indoxyl-b-D-Glucoside Agar (mEI) EPA 821-R-02-022 (September 2002) is an approved variation of Standard Method 9230C. The method is available at http://www.epa.gov/nerlcwww/1600sp02.pdf or EPA's Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW., Washington DC 20460. The holding time and temperature for ground water samples are specified in footnote 1 above, rather than as specified in Section 8 of EPA Method 1600.
9 Medium is available through IDEXX Laboratories, Inc., One IDEXX Drive, Westbrook, Maine 04092. Preparation and use of the medium is set forth in the article "Evaluation of Enterolert for Enumeration of Enterococci in Recreational Waters," by Budnick, G.E., Howard, R.T, and Mayo, D.R., 1996, Applied and Environmental Microbiology, 62:3881-3884.
Alternative for Analytical Testing for Source Monitoring Methods Water |
||||
Organism |
Methodology |
SM 21st Edition1 |
SM Online2 |
Other |
E. coli |
Colilert |
9223 B |
9223 B-97 |
|
Colisure |
9223 B |
9223 B-97 |
||
Colilert-18 |
9223 B |
9223 B-97 |
||
Readycult® |
||||
Colitag |
Readycult®3 |
|||
Chromocult® |
Modified ColitagT4 |
|||
Chromocult®5 | ||||
Enterococci |
Multiple-Tube Technique |
9230 B-04 |
Footnotes
1 Standard Methods for the Examination of Water and Wastewater, 21st edition (2005). Available from American Public Health Association, 800 I Street NW, Washington, DC 20001-3710.
2 Standard Methods Online are available at http://www.standardmethods.org. The year in which each method was approved by the Standard Methods Committee is designated by the last two digits in the method number. The methods listed are the only online versions that may be used.
3 Readycult® Method, "Readycult® Coliforms 100 Presence/Absence Test for Detection and Identification of Coliform Bacteria and Escherichia coli in Finished Waters," January, 2007. Version 1.1. Available from EMD Chemicals (affiliate of Merck KGaA,, Darmstadt, Germany), 480 S. Democrat Road, Gibbstown, NJ 08027-1297.
4 Modified ColitagTM Method. "Modified ColitagTM Test Method for the Simultaneous Detection of E. Coli and other Total Coliforms in Water (ATP D05-0035)," August 28, 2009. Available at http://www.nemi.gov or from CPI International, 5580 Skylane Boulevard, Santa Rosa, CA 95403.
5 Chromocult® Method, "Chromocult® Coliform Agar Presence/Absence Membrane Filter Test Method for Detection and Identification of Coliform Bacteria and Escherichia coli in Finished Waters," November, 2000. Version 1.0. EMD Chemicals (affiliate of Merck KGaA, Darmstadt, Germany), 480 S. Democrat Road, Gibbstown, NJ 08027-1297.
TABLE 0400-45-01-.14(10)(b)
Turbidity4 |
Nephelometric Method NephelometricMethod Great Lakes Instruments Hach FilterTrak |
2130 B 180.11 Method 22 101333 |
Footnotes
1. "Methods for the Determination of Inorganic Substances in Environmental Samples'', EPA/600/R-93/100, August 1993. Available at NTIS, PB94-121811.
2. GLI Method 2, "Turbidity'', November 2, 1992, Great Lakes Instruments, Inc., 8855 North 55th Street, Milwaukee, Wisconsin 53223.
3. A description of the Hach FilterTrak Method 10133, "Determination of Turbidity by Laser Nephelometry,'' January 2000, Revision 2.0, can be obtained from; Hach Co., P.O. Box 389, Loveland, CO 80539-0389, telephone: 800-227-4224.
4. Styrene divinyl benzene beads (e.g. AMCO-AEPA-1 or equivalent) and stabilized formazin (e.g. Hach StablCal TM or equivalent) are acceptable substitutes for formazin.
TABLE 0400-45-01-.14(10)(b) 1.
Contaminant |
Methodology |
SM 21 st Edition1 |
Other |
Turbidity |
Nephelometric Method |
2130 B |
|
Laser Nephelometry (on-line) |
Mitchell M52712 |
||
LED Nephelometry (on-line) |
Mitchell M53313 |
||
LED Nephelometry (on-line) |
AMI Turbiwell 4 |
||
LED Nephelometry (on-line) |
Orion AQ4500 5 |
Footnotes
1 Standard Methods for the Examination of Water and Wastewater, 21st edition (2005). Available from American Public Health Association, 800 I Street NW, Washington, DC 20001- 3710
2 Mitchell Method M5271, Revision 1.1. "Determination of Turbidity by Laser Nephelometry," March 5, 2009. Available at http://www.nemi.gov or from Leck Mitchell, Ph.D., PE, 656 Independence Valley Dr., Grand Junction, CO 81507.
3 Mitchell Method M5331, Revision 1.1. "Determination of Turbidity by LED Nephelometry," March 5, 2009. Available at http://www.nemi.gov or from Leck Mitchell, Ph.D., PE, 656 Independence Valley Dr., Grand Junction, CO 81507.
4 AMI Turbiwell, "Continuous Measurement of Turbidity Using a SWAN AMI Turbiwell Turbidimeter," August 2009. Available at http://www.nemi.gov or from Markus Bernasconi, SWAN Analytische Instrumente AG, Studbachstrasse 13, CH-8340 Hinwil, Switzerland.
5 Orion Method AQ4500, Revision 1.0. "Determination of Turbidity by LED Nephelometry," May 8, 2009. Available at http://www.nemi.gov or from Thermo Scientific, 166 Cummings Center, Beverly, MA 01915, http://www.thermo.com.
Inorganic Contaminants Analytical Methods TABLE 0400-45-01-.14(10)(c)
Contaminant |
Methodology 13 |
EPA |
ASTM 3 |
SM 4 (18th, 19th ed.) |
SM 4 (20th ed.) |
SM Online 22 |
Other |
1. Alkalinity |
Titrimetric Electrometric titration |
D1067-92, 02 B |
2320 B |
2320 B |
2320 B-97 I-1030-85 5 |
||
2. Antimony |
Inductively Coupled Plasma (ICP)-Mass Spectrometry |
200.82 |
|||||
Hydride-Atomic Absorption |
D3697-92, 02 |
||||||
Atomic Absorption;Platform |
200.92 |
||||||
Atomic Absorption; Furnace |
3113 B |
3113 B-99 |
|||||
3. Arsenic |
ICP-Mass Spectrometry |
200.82 |
|||||
Atomic Absorption;Platform |
200.92 |
||||||
Atomic Absorption; Furnace |
D2972-97, 03 C |
3113 B |
3113 B-99 |
||||
Hydride Atomic Absorption |
D2972-97, 03 B |
3114 B |
3114 B-97 |
||||
4. Asbestos |
Transmission Electron Microscopy |
100.19 |
|||||
Transmission Electron |
100.210 |
||||||
Microscopy |
|||||||
5. Barium |
Inductively Coupled Plasma |
200.72 |
3120 B |
3120 B |
3120 B-99 |
||
ICP-Mass Spectrometry |
200.82 |
||||||
Atomic Absorption; Direct |
3111D |
3111 D-99 |
|||||
Atomic Absorption; Furnace |
3113 B |
3113 B-99 |
|||||
6. Beryllium |
Inductively Coupled Plasma |
200.72 |
3120 B |
3120 B |
3120 B-99 |
||
ICP-Mass Spectrometry |
200.82 |
||||||
Atomic Absorption;Platform |
200.92 |
||||||
Atomic Absorption; Furnace |
D3645-97, 03 B |
3113 B |
3113 B-99 |
||||
7. Cadmium |
Inductively Coupled Plasma |
200.72 |
|||||
ICP-Mass Spectrometry |
200.82 |
||||||
Atomic Absorption;Platform |
200.92 |
||||||
Atomic Absorption; Furnace |
3113 B |
3113 B-99 |
|||||
8. Calcium |
EDTA titrimetric |
D511-93, 03 A |
3500-Ca D |
3500-Ca B |
3500-Ca B-97 |
||
Atomic Absorption; Direct Aspiration. |
D511-93, 03 B |
3111 B |
3111 B-99 |
||||
Inductively Coupled Plasma |
200.72 |
3120 B |
3120 B |
3120 B-99 |
|||
Ion Chromatography |
D6919-03 |
||||||
9. Chromium |
Inductively Coupled Plasma |
200.72 |
3120 B |
3120 B |
3120 B-99 |
||
ICP-Mass Spectrometry |
200.82 |
||||||
Atomic Absorption;Platform |
200.92 |
||||||
Atomic Absorption; Furnace |
3113 B |
3113 B-99 |
|||||
10. Copper |
Atomic Absorption; Furnace |
D1688-95, 02 C |
3113 B |
3113 B-99 |
|||
Atomic Absorption; Direct |
D1688-95, 02 A |
3111 B |
3111 B-99 |
||||
Aspiration. |
|||||||
Inductively Coupled Plasma |
200.72 |
3120 B |
3120 B |
3120 B-99 |
|||
ICP-Mass spectrometry |
200.82 |
||||||
Atomic Absorption;Platform |
200.92 |
||||||
11.Conductivity |
Conductance |
D1125-95 (Reapproved 1999) A |
2510 B |
2510 B |
2510 B-97 |
||
12. Cyanide |
Manual Distillation followed by Spectrophotometric, Amenable. Spectro-photometric Manual. Spectro-photometric Semi-automated. |
335.46 |
D2036-98 A D2036-98 B D2036-98 A |
4500-CN- C 4500-CN- G 4500-CN-E |
4500-CN- C 4500-CN-G 4500-CN-E |
4500-CN- G-99 4500-CN- E-99 |
I-3300-855 |
Selective Electrode |
4500-CN- F |
4500-CN- F |
4500-CN- F-99 |
||||
UV, Distillation, Spectrophotometric. |
Kelada-01 16 |
||||||
Micro Distillation, Flow Inj ection, Spectrophotometric. |
QuikChem 10-204-00-1-X17 |
||||||
Ligand Exchange and Amperometry19 |
D6888-04 |
OIA-1677, DW19 |
|||||
13. Fluoride |
Ion Chromatography |
300.06, 300.118 |
D4327-97, 03 |
4110 B |
4110 B |
4110 B-00 |
|
Manual Distill; Color. SPADNS. |
4500-F- B, D |
4500-F- B, D |
4500-F- B, D- 97 |
||||
Manual Electrode |
D1179-93, 99 B |
4500-F- C |
4500-F- C |
4500-F- C-97 |
|||
Automated Electrode |
380-75WE11 |
||||||
Automated Alizarin |
4500-F- E |
4500-F- E |
4500-F- E-97 |
129-71W11 |
|||
Capillary Ion Electrophoresis |
D6508, Rev. 222 |
||||||
14. Lead |
Atomic Absorption; Furnace |
D3559-96, 03 D |
3113 B |
3113 B-99 |
|||
ICP-Mass spectrometry Atomic Absorption;Platform Differential Pulse Anodic |
200.82 200.92 |
Method |
|||||
Stripping Voltametry |
100115 |
||||||
15. Magnesium |
Atomic Absorption ICP |
200.72 |
D511-93, 03 B |
3111 B 3120 B |
3120 B |
3111 B-99 3120 B-99 |
|
Complexation Titrimetric Methods |
D511-93, 03 A |
3500-Mg E |
3500-Mg B |
3500-Mg B-97 |
|||
Ion Chromatography |
D6919-03 |
||||||
16. Mercury |
Manual, Cold Vapor |
245.12 |
D3223-97, 02 |
3112 B |
3112 B-99 |
||
Automated, Cold Vapor |
245.21 |
||||||
ICP-Mass Spectrometry |
200.82 |
||||||
17. Nickel |
Inductively Coupled Plasma |
200.72 |
3120 B |
3120 B |
3120 B-99 |
||
ICP-Mass Spectrometry Atomic Absorption;Platform |
200.82 200.92 |
||||||
Atomic Absorption; Direct |
3111 B |
3111 B-99 |
|||||
Atomic Absorption; Furnace |
3113 B |
3113 B-99 |
|||||
18. Nitrate |
Ion Chromatography |
300.06 300.12 |
D4327-97, 03 |
4110 B |
4110 B |
4110 B-00 |
B-10118 |
Automated Cadmium Reduction |
353.26 |
D3867-90 A |
4500-NO3- F |
4500-NO3-F |
4500-NO3- F-00 |
||
Ion Selective Electrode |
4500-NO3- D |
4500-NO3-D |
4500-NO3- D -00 |
6017 |
|||
Manual Cadmium Reduction |
D3867-90 B |
4500-NO3- E |
4500-NO3-E |
4500-NO3- E-00 |
|||
Capillary Ion Electrophoresis |
D6508, Rev 222 |
||||||
19. Nitrite |
Ion Chromatography |
300.06 300.12 |
D4327-97, 03 |
4110 B |
4110 B |
4110 B-00 |
B-1011 8 |
Automated Cadmium Reduction |
353.26 |
D3867-90 A |
4500-NO3- F |
4500-NO3-F |
4500-NO3- F -00 |
||
Manual Cadmium Reduction |
D3867-90 B |
4500-NO3E |
4500-NO3-E |
4500-N O3- E-00 |
|||
Spectrophotometric |
4500-NO2 -B |
4500-NO2-B |
4500-NO2-B-00 |
||||
Capillary Ion Electrophoresis |
D6508, Rev. 222 |
||||||
20. Ortho-phosphate12 |
Colorimetric, Automated, Ascorbic Acid. |
365.16 |
4500-P F |
4500-P F |
|||
Colorimetric, ascorbic acid, single reagent. |
D515-88 A |
4500-P E |
4500-P E |
||||
Colorimetric Phosphomolybdate; Automated-seg- |
I-1601-855 I-2601-905 |
||||||
mented flow; Automated Discrete |
I-2598-855 |
||||||
Ion Chromatography |
300.06 300.118 |
D4327-97, 03 |
4110 B |
4110 B |
4110 B-00 |
||
Capillary Ion Electro-phoresis. |
D6508, Rev. 222 |
||||||
21. pH |
Electrometric |
150.1, 150.21 |
D1293-95, 99 |
4500-H+ B |
4500-H+ B |
4500-H+ B - 00 |
|
22. Selenium |
Hydride-Atomic Absorption |
D3859-98, 03 A |
3114 B |
3114 B-97 |
|||
ICP-Mass Spectrometry |
200.82 |
||||||
Atomic Absorption;Platform |
200.92 |
||||||
Atomic Absorption; Furnace |
D3859-98, 03 B |
3113 B |
3113 B-99 |
||||
23. Silica |
Colorimetric, Molybdate Blue Automated-segmentedFlow |
I-1700-855 I-2700-855 |
|||||
Colorimetric |
D859-94, 00. |
||||||
Molybdosilicate . |
4500-Si D |
4500-SiO2 C |
4500-SiO2 C-97 |
||||
Heteropoly blue |
4500-Si E |
4500-SiO2 D |
4500-SiO2 D-97 |
||||
Automated for Molyb-date-reactive Silica. |
4500-Si F |
4500-SiO2 E |
4500-SiO2 E-97 |
||||
Inductively Coupled Plasma |
200.72 |
3120 B |
3120 B |
3120 B-99 |
|||
24. Sodium |
Inductively Coupled Plasma |
200.72 |
|||||
Atomic Absorption; Direct Aspiration. |
3111 B |
3111 B-99 |
|||||
Ion Chromatography |
D6919-03 |
||||||
25. Temperature |
Thermometric |
2550 |
2550 |
2550-00 |
|||
26. Thallium |
ICP-Mass Spectrometry |
200.82 |
|||||
Atomic Absorption;Platform |
200.92 |
Footnotes
1. "Methods for Chemical Analysis of Water and Wastes,'' EPA/600/4-79/020, March 1983. Available at NTIS, PB84-128677.
2. "Methods for the Determination of Metals in Environmental Samples-Supplement I,'' EPA/600/R-94/111, May 1994. Available at NTIS, PB95-125472.
3. Annual Book of ASTM Standards, 1994, 1996, 1999, or 2003, Vols. 11.01 and 11.02, ASTM International; any year containing the cited version of the method may be used. The previous versions of D1688-95A, D1688-95C (copper), D3559-95D (lead), D1293-95 (pH), D1125-91A (conductivity) and D859-94 (silica) are also approved. These previous versions D1688-90A, C; D3559-90D, D1293-84, D1125-91A and D859-88, respectively are located in the Annual Book of ASTM Standards, 1994, Vol. 11.01. Copies may be obtained from ASTM International, 100 Barr Harbor Drive, West Conshohocken, PA 19428.
4. Standard Methods for the Examination of Water and Wastewater, 18th edition (1992), 19th edition (1995), or 20th edition (1998). American Public Health Association, 1015 Fifteenth Street, NW., Washington, DC 20005. The cited methods published in any of these three editions may be used, except that the versions of 3111 B, 3111 D, 3113 B and 3114 B in the 20th edition may not be used.
5. Method I-2601-90, Methods for Analysis by the U.S. Geological Survey National Water Quality Laboratory-Determination of Inorganic and Organic Constituents in Water and Fluvial Sediment, Open File Report 93-125, 1993; For Methods I-1030-85; I-1601-85; I-1700-85; I-2598-85; I-2700-85; and I-3300-85 See Techniques of Water Resources Investigation of the U.S. Geological Survey, Book 5, Chapter A-1, 3rd edition., 1989; Available from Information Services, U.S. Geological Survey, Federal Center, Box 25286, Denver, CO 80225-0425.
6. "Methods for the Determination of Inorganic Substances in Environmental Samples,'' EPA/600/R-93/100, August 1993. Available at NTIS, PB94-120821.
7. The procedure shall be done in accordance with the Technical Bulletin 601 "Standard Method of Test for Nitrate in Drinking Water," July 1994, PN 221890-001, Analytical Technology, Inc. Copies may be obtained from ATI Orion, 529 Main Street, Boston, MA 02129.
8. Method B-1011, "Waters Test Method for Determination of Nitrite/Nitrate in Water Using Single Column Ion Chromatography,'' August 1987. Copies may be obtained from Waters Corporation, Technical Services Division, 34 Maple Street, Milford, MA 01757, Telephone: 508/482-2131, Fax: 508/482-3625.
9. Method 100.1, "Analytical Method For Determination of Asbestos Fibers in Water,'' EPA/600/4-83/043, EPA, September 1983. Available at NTIS, PB83-260471.
10. Method 100.2, "Determination of Asbestos Structure Over 10-±m In Length In Drinking Water,'' EPA/600/R-94/134, June 1994. Available at NTIS, PB94-201902.
11. Industrial Method No. 129-71W, "Fluoride in Water and Wastewater,'' December 1972, and Method No. 380-75WE, "Fluoride in Water and Wastewater,'' February 1976, Technicon Industrial Systems. Copies may be obtained from Bran & Luebbe, 1025 Busch Parkway, Buffalo Grove, IL 60089.
12. Unfiltered, no digestion or hydrolysis.
13. Because MDLs reported in EPA Methods 200.7 and 200.9 were determined using a 2x preconcentration step during sample digestion, MDLs determined when samples are analyzed by direct analysis (i.e., no sample digestion) will be higher. For direct analysis of cadmium and arsenic by Method 200.7, and arsenic by Method 3120 B, sample preconcentration using pneumatic nebulization may be required to achieve lower detection limits. Preconcentration may also be required for direct analysis of antimony, lead, and thallium by Method 200.9; antimony and lead by Method 3113 B; and lead by Method D3559-90D, unless multiple in-furnace depositions are made.
14. If ultrasonic nebulization is used in the determination of arsenic by Methods 200.7, 200.8, or SM 3120 B, the arsenic must be in the pentavalent state to provide uniform signal response. For Methods 200.7 and 3120 B, both samples and standards must be diluted in the same mixed acid matrix concentration of nitric and hydrochloric acid with the addition of 100 ± L of 30% hydrogen peroxide per 100 mL of solution. For direct analysis of arsenic with Method 200.8 using ultrasonic nebulization, samples and standards must contain 1 mg/L of sodium hypochlorite.
15. The description for Method Number 1001 for lead is available from Palintest, LTD, 21 Kenton Lands Road, P.O. Box 18395, Erlanger, KY 41018. Or from the Hach Company, P.O. Box 389, Loveland, CO 80539.
16. The description for the Kelada-01 Method, "Kelada Automated Test Methods for Total Cyanide, Acid Dissociable Cyanide, And Thiocyanate,'' Revision 1.2, August 2001, EPA # 821-B01-009 for cyanide is available from the National Technical Information Service (NTIS), PB 2001-108275, 5285 Port Royal Road, Springfield, VA 22161. The toll free telephone number is 800-553-6847. Note: A 450-W UV lamp may be used in this method instead of the 550-W lamp specified if it provides performance within the quality control (QC) acceptance criteria of the method in a given instrument. Similarly, modified flow cell configurations and flow conditions may be used in the method, provided that the QC acceptance criteria are met.
17. The description for the QuikChem Method 10-204-00-1-X, "Digestion and distillation of total cyanide in drinking and wastewaters using MICRO DIST and determination of cyanide by flow injection analysis,'' Revision 2.1, November 30, 2000, for cyanide is available from Lachat Instruments, 6645 W. Mill Rd., Milwaukee, WI 53218. Telephone: 414-358-4200.
18. "Methods for the Determination of Organic and Inorganic Compounds in Drinking Water,'' Vol. 1, EPA 815-R-00-014, August 2000. Available at NTIS, PB2000-106981.
19. Method OIA-1677, DW "Available Cyanide by Flow Injection, Ligand Exchange, and Amperometry,'' January 2004. EPA-821-R-04-001, Available from ALPKEM, A Division of OI Analytical, P.O. Box 9010, College Station, TX 77842-9010.
20. Sulfide levels below those detected using lead acetate paper may produce positive method interferences. Test samples using a more sensitive sulfide method to determine if a sulfide in-terference is present, and treat samples accordingly.
21. Standard Methods Online are available at http://www.standardmethods.org. The year in which each method was approved by the Standard Methods Committee is designated by the last two digits in the method number. The methods listed are the only online versions that may be used.
22. Method D6508, Rev. 2, "Test Method for Determination of Dissolved Inorganic Anions in Aqueous Matrices Using Capillary Ion Electrophoresis and Chromate Electrolyte,'' available from Waters Corp, 34 Maple St, Milford, MA, 01757, Telephone: 508/482-2131, Fax: 508/482-3625.
TABLE 0400-45-01-.14(10)(c) 1.
Contaminant |
Methodology |
EPA Method |
SM 21 st Edition1 |
SM Online2 |
ASTM 3 |
Other |
Alkalinity |
Titrimetric |
2320 B |
||||
Antimony |
Hydride - Atomic Absorption |
D 3697-07 |
||||
Atomic Absorption; Furnace |
3113 B |
|||||
Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES) |
200.5, Revision 4.24 |
|||||
Arsenic |
Atomic Absorption; Furnace |
3113 B |
D 2972-08 C |
|||
Hydride Atomic Absorption |
3114 B |
D 2972-08 B |
||||
Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES) |
200.5, Revision 4.2 |
|||||
Barium |
Inductively Coupled Plasma |
3120 B |
||||
Atomic Absorption; Direct |
3111 D |
|||||
Atomic Absorption; Furnace |
3113 B |
|||||
Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES) |
200.5, Revision 4.2 |
|||||
Beryllium |
Inductively Coupled Plasma |
3120 B |
||||
Atomic Absorption; Furnace |
3113 B |
D 3645-08 B |
||||
Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES) |
200.5, Revision 4.2 |
|||||
Cadmium |
Atomic Absorption; Furnace |
3113 B |
||||
Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES) |
200.5, Revision 4.2 |
|||||
Calcium |
EDTA titrimetric |
3500-Ca B |
D 511-09 A |
|||
Atomic Absorption; Direct Aspiration |
3111 B |
D 511-09 B |
||||
Inductively Coupled Plasma |
3120 B |
|||||
Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES) |
200.5, Revision 4.2 |
|||||
Chromiuim |
Inductively Coupled Plasma |
3120 B |
||||
Atomic Absorption; |
3113 B |
|||||
Furnace |
||||||
Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES) |
200.5, Revision 4.2 |
|||||
Copper |
Atomic Absorption; Furnace |
3113 B |
D 1688-07 C |
|||
Atomic Absorption; Direct Aspiration |
3111 B |
D 1688-07 A |
||||
Inductively Coupled Plasma |
3120 B |
|||||
Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES) |
200.5, Revision 4.2 |
|||||
Conductivity |
Conductance |
2510 B |
||||
Cyanide |
Manual Distillation followed by: |
D2036-06 A |
||||
Spectro-photometric, Amenable |
4500-CN -G |
D2036-06 B |
||||
Spectro-photometric Manual |
4500-CN -E |
D2036-06 A |
||||
Selective Electrode |
4500-CN -F |
|||||
Gas Chromatography/Mass Spectrometry Headspace |
ME355.01 5 |
|||||
Fluoride |
Ion Chromatography |
4110 B |
||||
Manual Distillation; Colorimetric SPADNS |
4500-F -B, D |
|||||
Manual Electrode |
4500-F - C |
D1179-04 B |
||||
Automated Alizarin |
4500-F - E |
|||||
Lead |
Atomic Absorption; Furnace |
3113 B |
D 3559-08 D |
|||
Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES) |
200.5, Revision 4.2 |
|||||
Magnesium |
Atomic Absorption |
3111 B |
D 511-09 B |
|||
Inductively Coupled Plasma |
3120 B |
|||||
Complexation Titrimetric Methods |
3500-Mg B |
D 511-09 A |
||||
Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES) |
200.5, Revision 4.2 |
|||||
Mercury |
Manual, Cold Vapor |
3112 B |
||||
Nickel |
Inductively Coupled Plasma |
3120 B |
||||
Atomic Absorption; Direct |
3111 B |
|||||
Atomic Absorption; Furnace |
3113 B |
|||||
Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES) |
200.5, Revision 4.2 |
|||||
Nitrate |
Ion Chromatography |
4110 B |
||||
Automated Cadmium Reduction |
4500-NO3- F |
|||||
Manual Cadmium Reduction |
4500-NO3- E |
|||||
Ion Selective Electrode |
4500-NO2- B |
|||||
Reduction/Colorimetric |
Systea Easy (1 - Reagent)6 |
|||||
Nitrite |
Ion Chromatography |
4110 B |
||||
Automated Cadmium Reduction |
4500-NO3- F |
|||||
Manual Cadmium Reduction |
4500-NO3- E |
|||||
Spectrophotometric |
4500-NO2- B |
|||||
Reduction/Colorimetric |
Systea Easy (1 - Reagent)6 |
|||||
Orthophosphate |
Ion Chromatography |
4110 B |
||||
Colorimetric, ascorbic acid, single reagent |
4500-P E |
4500-P E-99 |
||||
Colorimetric, Automated, Ascorbic Acid |
4500-P F |
4500-P F-99 |
||||
pH |
Electrometric |
4500-H + B |
||||
Selenium |
Hydride-Atomic Absorption |
3114 B |
D 3859-08 A |
|||
Atomic Absorption; Furnace |
3113 B |
D 3859-08 B |
||||
Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES) |
200.5, Revision 4.2 |
|||||
Silica |
Colorimetric |
D859-05 |
||||
Molybdosilicate |
4500-SiO2 C |
|||||
Heteropoly blue |
4500-SiO2 D |
|||||
Automated for |
4500- |
|||||
Molybdate-reactive Silica |
SiO2 E |
|||||
Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES) |
200.5, Revision 4.2 |
|||||
Inductively Coupled Plasma |
3120 B |
|||||
Sodium |
Atomic Absorption; Direct Aspiration |
3111 B |
||||
Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES) |
200.5, Revision 4.2 |
|||||
Temperature |
Thermometric |
2550 |
Footnotes
1 Standard Methods for the Examination of Water and Wastewater, 21st edition (2005). Available from American Public Health Association, 800 I Street NW, Washington, DC 20001- 3710
2 Standard Methods Online are available at http://www.standardmethods.org. The year in which each method was approved by the Standard Methods Committee is designated by the last two digits in the method number. The methods listed are the only online versions that may be used.
3 Available from ASTM International, 100 Barr Harbor Drive, West Conshohocken, PA 19428-2959 or http://astm.org. The methods listed are the only alternative versions that may be used.
4 EPA Method 200.5, Revision 4.2. "Determination of Trace Elements in Drinking Water by Axially Viewed Inductively Coupled Plasma-Atomic Emission Spectrometry." 2003. EPA/600/R-06/115. (Available at http://www.epa.gov/nerlcwww.ordmeth.htm. )
5 Method D99-003, Revision 3.0. "Free Chlorine Species (HOCl- and OCl-) by Test Strip," November 21, 2003. Available from Industrial Test Systems, Inc., 1875 Langston St., Rock Hill, SC 29730.
6 Systea Easy (1 - Reagent). "Systea Easy (1 - Reagent) Nitrate Method," February 4, 2009. Available at http://www.nemi.gov or from Systea Scientific, LLC., 900 Jorie Blvd., Suite 35, Oak Brook, IL 60523.
TABLE 0400-45-01-.14(10)(d) Detection Limits for Inorganic Contaminants
Contaminant |
MCL (mg/l) |
Methodology |
Detection limit (mg/1) |
Antimony |
0.006 |
Atomic Absorption Furnace |
0.003 |
Atomic Absorption: Platform |
0.00085 |
||
ICP-Mass Spectrometry |
0.0004 |
||
Hydride-Atomic Absorption |
0.001 |
||
Arsenic |
0.016 |
Atomic Absorption Furnace |
0.001 |
Atomic Absorption: Platform |
|||
Stabilized Temperature |
70.0005 |
||
Atomic Absorption: Gaseous Hydride |
0.001 |
||
ICP-Mass Spectrometry |
80.0014 |
||
Asbestos |
7 MFL |
Transmission Electron Microscopy |
0.01 MFL |
Barium |
2 |
Atomic Absorption; furnace technique |
0.002 |
Atomic Absorption; Direct Aspiration |
0.1 |
||
Inductively Coupled Plasma |
0.002(0.001) |
||
Beryllium |
0.004 |
Atomic Absorption; Furnace |
0.00025 |
Atomic Absorption; Platform |
0.00002 |
||
Inductively Coupled Plasma |
0.0003 |
||
ICP-Mass Spectrometry |
0.0003 |
||
Cadmium |
0.005 |
Atomic Absorption; furnace technique |
0.0001 |
Inductively Coupled Plasma |
0.001 |
||
Chromium |
0.1 |
Atomic Absorption; furnace technique |
0.001 |
Inductively Coupled Plasma |
0.007(0.001) |
||
Cyanide |
0.2 |
Distillation, Spectrophotometric3 |
0.02 |
Distillation, Automated Spectrophotometric3 |
0.005 |
||
Distillation, Selective Electrode |
0.05 |
||
Distillation, Amenable, Spectrophotometric4 |
0.02 |
||
Mercury |
0.002 |
Manual Cold Vapor Technique |
0.0002 |
Automated Cold Vapor Technique |
0.0002 |
||
Nickel |
0.1 |
Atomic Absorption; Furnace |
0.001 |
Atomic Absorption; Platform |
0.00065 |
||
Inductively Coupled Plasma2 |
0.005 |
||
ICP-Mass Spectrometry |
0.0005 |
||
Nitrate |
10 (as N) |
Manual Cadmium Reduction |
0.01 |
Automated Hydrazine Reduction |
0.01 |
||
Automated Cadmium Reduction |
0.05 |
||
Ion Selective Electrode |
1 |
||
Ion Chromatography |
0.01 |
||
Nitrite |
1 (as N) |
Spectrophotometric |
0.01 |
Automated Cadmium Reduction |
0.05 |
||
Manual Cadmium Reduction |
0.01 |
||
Ion Chromatography |
0.004 |
||
Selenium |
0.05 |
Atomic Absorption; furnace |
0.002 |
Atomic Absorption; gaseous hydride |
0.002 |
||
Thallium |
0.002 |
Atomic Absorption; Furnace |
0.001 |
Atomic Absorption; Platform |
0.00075 |
||
ICP-Mass Spectrometry |
0.0003 |
Footnotes 1 MFL=million fibers per liter > 10 microns.
2 Using a 2X preconcentration step as noted in Method 200.7 Lower method detection limits (MDLs) may be achieved using a 4X preconcentration.
3 Screening method for total cyanides.
4 Measures "free" cyanides.
5 Lower MDLs are reported using stabilized temperature graphite furnace atomic absorption.
6 The value for arsenic is effective January 23, 2006. Until then the MCL is 0.05 mg/L.
7. The MDL reported for EPA method 200.9 (Atomic Absorption; Platform-Stabilized Temperature) was determined using a 2X concentration step during sample digestion. The MCL determined for samples analyzed using direct analyses (i.e, no sample digestion) will be higher. Using multiple depositions, EPA 200.9 is capable of obtaining MDL of 0.0001 mg/L.
8 Using selective ion monitoring, EPA Method 200.8 (ICP-MS) is capable of obtaining a MDL of 0.0001 mg/L.
Contaminant |
Acceptance limit |
Antimony |
±30 at >=0.006 mg/l |
Arsenic |
±30 at >=0.003 mg/l |
Asbestos |
2 standard deviations based on study statistics |
Barium |
±15% at >=0.15 mg/l |
Beryllium |
±15% at >=0.001 mg/l |
Cadmium |
±20% at >=0.002 mg/l |
Chromium |
±15% at >=0.01 mg/l |
Copper |
±10% at >=0.050 mg/l1 |
Cyanide |
±25% at >=0.1 mg/l |
Fluoride |
±10% at >=1 to 10 mg/l |
Lead |
± 30% >=0.005 mg/l2 |
Mercury |
±30% at >=0.0005 mg/l |
Nickel |
±15% at >=0.01 mg/l |
Nitrate |
±10% at >=0.4 mg/l |
Nitrite |
±15% at >=0.4 mg/l |
Selenium |
±20% at >=0.01 mg/l |
Thallium |
±30% at >=0.002 mg/l |
TABLE 0400-45-01-.14(10)(f)
Contaminant |
Preservative1 |
Container2 |
Time3 |
Antimony |
HNO3 |
P or G |
6 months |
Arsenic |
Conc HNO3to pH<2 |
P or G |
6 months |
Asbestos |
4°C |
P or G |
48 hours4 |
Barium |
HNO3 |
P or G |
6 months |
Beryllium |
HNO3 |
P or G |
6 months |
Cadmium |
HNO3 |
P or G |
6 months |
Chromium |
HNO3 |
P or G |
6 months |
Cyanide |
4°C, NaOH |
P or G |
14 days |
Fluoride |
None |
P or G |
1 month |
Mercury |
HNO3 |
P or G |
28 days |
Nickel |
HNO3 |
P or G |
6 months |
Nitrate |
4°C |
P or G |
48 hours5 |
Nitrate-Nitrite 6 |
H2SO4 |
P or G |
28 days |
Nitrite |
4°C |
P or G |
48 hours |
Selenium |
HNO3 |
P or G |
6 months |
Thallium |
HNO3 |
P or G |
6 months |
Footnotes
1. For cyanide determinations samples must be adjusted with sodium hydroxide to pH 12 at the time off collection. When chilling is indicated the sample must be shipped and stored at 4°C or less. Acidification of nitrate or metals samples may be with a concentrated acid or a dilute (50% by volume) solution of the applicable concentrated acid. Acidification of samples for metals analysis is encouraged and allowed at the laboratory rather than at the time of sampling provided the shipping time and other instructions in Section 8.3 of EPA Methods 200.7 or 200.8 or 200.9 are followed.
2. P=plastic, hard or soft; G=glass, hard or soft.
3. In all cases samples should be analyzed as soon after collection as possible. Follow additional (if any) information on preservation, containers or holding times that is specified in method.
4. Instructions for containers, preservation procedures and holding times as specified in Method 100.2 must be adhered to for all compliance analyses including those conducted with Method 100.1.
5. If the sample is chlorinated, the holding time for an unacidified sample kept at 4°C is extended to 14 days.
6. Nitrate-Nitrite refers to a measurement of total nitrate.
TABLE 0400-45-01-.14(10)(g)
Contaminant |
EPA method1 |
Standard Methods |
ASTM |
Other |
1. Benzene |
502.2, 524.2 |
|||
2. Carbon tetrachloride |
502.2, 524.2, 551.1 |
|||
3. Chlorobenzene |
502.2, 524.2 |
|||
4. 1,2-Dichlorobenzene |
502.2, 524.2 |
|||
5. 1,4-Dichlorobenzene |
502.2, 524.2 |
|||
6. 1,2-Dichloroethane |
502.2, 524.2 |
|||
7. cis-Dichloroethylene |
502.2, 524.2 |
|||
8. trans-Dichloroethylene |
502.2, 524.2 |
|||
9. Dichloromethane |
502.2, 524.2 |
|||
10. 1,2-Dichloropropane |
502.2, 524.2 |
|||
11. Ethylbenzene |
502.2, 524.2 |
|||
12. Styrene |
502.2, 524.2 |
|||
13. Tetrachloroethylene |
502.2, 524.2, 551.1 |
|||
14. 1,1,1-Trichloroethane |
502.2, 524.2, 551.1 |
|||
15. Trichloroethylene |
502.2, 524.2, 551.1 |
|||
16. Toluene |
502.2, 524.2 |
|||
17. 1,2,4-Trichlorobenzene |
502.2, 524.2 |
|||
18. 1,1-Dichloroethylene |
502.2, 524.2 |
|||
19. 1,1,2-Trichloroethane |
502.2, 524.2, 551.1 |
|||
20. Xylenes (total) |
502.2, 524.2 |
|||
21. Vinyl chloride |
502.2, 524.2 |
|||
22. 2,3,7,8-TCDD (dioxin) |
1613 |
|||
23. 2,4-D4 (as acid, salts and esters) |
515.2, 555, 515.1, 515.3, 515.4 |
D5317-93,98 (Reapproved 2003) |
||
24. 2,4,5-TP 4 (Silvex) |
515.2, 555, 515.1, 515.3, 515.4 |
D5317-93,98 (Reapproved 2003 |
||
25. Alachlor2 |
507, 525.2, 508.1, 505, 551.1 |
|||
26. Atrazine2 |
507, 525.2, 508.1, 505, 551.1 |
Syngenta5 AG- 625 |
||
27. Benzo(a)pyrene |
525.2, 550, 550.1 |
|||
28. Carbofuran |
531.1, 531.2 |
6610 |
||
29. Chlordane |
508, 525.2, 508.1, 505 |
|||
30. Dalapon |
552.1, 515.1, 552.2, 515.3, 515.4, 552.3 |
|||
31. Di(2-ethylhexyl)adipate |
506, 525.2 |
|||
32. Di(2-ethylhexyl)phthalate |
506, 525.2 |
|||
33. Dibromochloropropane (DBCP) |
504.1, 551.1 |
|||
34. Dinoseb4 |
515.2, 555, 515.1, 515.3, 515.4 |
|||
35. Diquat |
549.2 |
|||
36. Endothall |
548.1 |
|||
37. Endrin |
508, 525.2, 508.1, 505, 551.1 |
|||
38. Ethylene dibromide (EDB) |
504.1, 551.1 |
|||
39. Glyphosate |
547 |
6651 |
||
40. Heptachlor |
508, 525.2, 508.1, 505, 551.1 |
|||
41. Heptachlor Epoxide |
508, 525.2, 508.1, 505, 551.1 |
|||
42. Hexachlorobenzene |
508, 525.2, 508.1, 505, 551.1 |
|||
43. Hexachlorocyclopentadiene |
508, 525.2, 508.1, 505, 551.1 |
|||
44. Lindane |
508, 525.2, 508.1, 505, 551.1 |
|||
45. Methoxychlor |
508, 525.2, 508.1, 505, 551.1 |
|||
46. Oxamyl |
531.1, 531.2 |
6610 |
||
47. PCBs3 (as decachlorobiphenyl) |
508A |
|||
48. PCBs3 (as Aroclors) |
508.1, 508, 525.2, 505 |
|||
49. Pentachlorophenol |
515.2, 525.2, 555, 515.1, 515.3, 515.4 |
D5317-93,98 (Reapproved 2003) |
||
50. Picloram4 |
515.2, 555, 515.1, 515.3, 515.4 |
D5317-93,98 (Reapproved 2003) |
||
51. Simazine2 |
507, 525.2, 508.1, 505, 551.1 |
|||
52. Toxaphene |
508, 508.1, 525.2, 505 |
Footnotes
1. Previously approved EPA methods remain available for compliance monitoring until June 1, 2001. EPA methods 502.2 Rev. 2.0, 505 Rev. 2.0, 507 Rev. 2.0, 508 Rev. 3.0, 531.1 Rev. 3.0 are in "Methods for the Determination of Organic Compounds in Drinking Water", December 1988, revised July 1991; methods 506 and 551 are in "Methods for the Determination of Organic Compounds in Drinking Water--Supplement I", July 1990; methods 515.2 Rev. 1.0 and 524.2 Rev. 4.0 are in "Methods for the Determination of Organic Compounds in Drinking Water--Supplement II,'' August 1992; and methods 504.1 Rev. 1.0, 508.1 Rev. 1.0, 525.2 Rev.1.0 are available from US EPA NERL, Cincinnati, OH 45268
2. Substitution of the detector specified in Method 505, 507, 508, or 508.1 for the purpose of achieving lower detection limits is allowed as follows: Either an electron capture or nitrogen phosphorus detector may be used provided all regulatory requirements and quality control criteria are met.
3. PCBs are qualitatively identified as Aroclors and measured for compliance purposes as decachlorobiphenyl. Users of Method 505 may have more difficulty in achieving the required detection limits than users of Methods 508.1, 525.2 or 508.
4. Accurate determination of the chlorinated esters requires hydrolysis of the sample as described in EPA Methods 515.1, 515.2, 515.3, 515.4, and 555 and ASTM Method D 5317-93, 98 (Reapproved 2003).
5. This method may not be used for the analysis of atrazine in any system where chlorine dioxide is used for drinking water treatment. In samples from all other systems, any result for atrazine generated by Method AG-625 that is greater than one-half the maximum contaminant level (MCL) (in other words, greater than 0.0015mg/L or 1.5µ/L) must be confirmed using another approved method for this contaminant and should use additional volume of the original sample collected for compliance monitoring. In instances where a result from Method AG-625 triggers such confirmatory testing, the confirmatory result is to be used to determine compliance.
TABLE A
The Organic Contaminants from table 0400-45-01-.14(10)(g) that have Alternative Testing Methods
Contaminant |
Methodology |
EPA Method |
SM 21stEdition1 |
SM Online2 |
Benzene |
Purge & Trap/Gas Chromatography/Mass Spectrometry (PT/GC/MS) |
524.33 |
||
Carbon tetrachloride |
PT/GC/MS |
524.3 |
||
Chlorobenzene |
PT/GC/MS |
524.3 |
||
1,2-Dichlorobenzene |
PT/GC/MS |
524.3 |
||
1,4-Dichlorobenzene |
PT/GC/MS |
524.3 |
||
1,2-Dichloroethane |
PT/GC/MS |
524.3 |
||
cis-Dichloroethylene |
PT/GC/MS |
524.3 |
||
trans-Dichloroethylene |
PT/GC/MS |
524.3 |
||
Dichloromethane |
PT/GC/MS |
524.3 |
||
1,2-Dichloropropane |
PT/GC/MS |
524.3 |
||
Ethylbenzene |
PT/GC/MS |
524.3 |
||
Styrene |
PT/GC/MS |
524.3 |
||
Tetrachloroethylene |
PT/GC/MS |
524.3 |
||
1,1,1-Trichloroethane |
PT/GC/MS |
524.3 |
||
Trichloroethylene |
PT/GC/MS |
524.3 |
||
Toluene |
PT/GC/MS |
524.3 |
||
1,2,4-Trichlorobenzene |
PT/GC/MS |
524.3 |
||
1,1-Dichloroethylene |
PT/GC/MS |
524.3 |
||
1,1,2-Trichloroethane |
PT/GC/MS |
524.3 |
||
Xylenes (total) |
PT/GC/MS |
524.3 |
||
Vinyl chloride |
PT/GC/MS |
524.3 |
||
Carbofuran |
High-performance liquid chromatography (HPLC) with post-column derivatization and fluorescence detection |
6610 B |
6610 B-04 |
|
Dalapon |
Ion Chromatography Electrospray Ionization Tandem Mass Spectrometry (IC-ESI-MS/MS) |
5574 |
6640 B |
6640 B-01 |
Dibromochloropropane (DBCP) |
PT/GC/MS |
524.3 |
||
Ethyl dibromide (EDB) |
PT/GC/MS |
524.3 |
||
Oxamyl |
High-performance liquid chromatography (HPLC) with post-column derivatization and fluorescence detection |
6610 B |
6610 B-04 |
|
Total Trihalomethanes |
PT/GC/MS |
524.3 |
Footnotes
1 Standard Methods for the Examination of Water and Wastewater, 21st edition (2005). Available from American Public Health Association, 800 I Street NW, Washington, DC 20001-3710
2. Standard Methods Online are available at http://www.standardmethods.org. The year in which each method was approved by the Standard Methods Committee is designated by the last two digits in the method number. The methods listed are the only online versions that may be used.
3. EPA Method 524.3, Version 1.0. "Measurement of Purgeable Organic Compounds in Water by Capillary Column Gas Chromatography/Mass Spectrometry," June 2009. EPA 815-B-09-009. Available at http://epa.gov/safewater/methods/analyticalmethods_ogwdw.html.
4. EPA Method 557. "Determination of Haloacetic Acids, Bromate, and Dalapon in Drinking Water by Ion Chromatography Electrospray Ionization Tandem Mass Spectrometry (IC-ESI-MS/MS)," September 2009, EPA 815-B-09-012. Available at http://epa.gov/safewater/methods/analyticalmethods_ogwdw.html.
TABLE 0400-45-01-.14(10)(g) 3.
Contaminant |
Acceptance limits (percent) |
DBCP |
+ 40 |
EDB |
+ 40 |
Alachlor |
+ 45 |
Atrazine |
+ 45 |
Benzo[a]pyrene |
2 standard deviations |
Carbofuran |
+ 45 |
Chlordane |
+ 45 |
Dalapon |
2 standard deviations |
Di(2-ethylhexyl)adipate |
2 standard deviations |
Di(2-ethylhexyl)phthalate |
2 standard deviations |
Dinoseb |
2 standard deviations |
Diquat |
2 standard deviations |
Endothall |
2 standard deviations |
Endrin |
+ 30 |
Glyphosate |
2 standard deviations |
Heptachlor |
+ 45 |
Heptachlor Epoxide |
+ 45 |
Hexachlorobenzene |
2 standard deviations. |
Hexachlorocyclopentadiene |
2 standard deviations |
Lindane |
+ 45 |
Methoxychlor |
+ 45 |
Oxamyl |
2 standard deviations |
PCBs (as Decachlorobiphenyl) |
0 - 200 |
Picloram |
2 standard deviations |
Simazine |
2 standard deviations |
Toxaphene |
+ 45 |
Aldicarb |
2 standard deviations |
Aldicarb sulfoxide |
2 standard deviations |
Aldicarb sulfone |
2 standard deviations. |
Pentachlorophenol |
+ 50 |
2,3,7,8-TCDD (Dioxin) |
2 standard deviations |
2,4-D |
+ 50 |
2,4,5-TP (Silvex) |
+ 50 |
TABLE 0400-45-01-.14(10)(g) 4.(ii)
Aroclor |
Detection Limit (mg/l) |
1016 |
0.00008 |
1221 |
0.02 |
1232 |
0.0005 |
1242 |
0.0003 |
1248 |
0.0001 |
1254 |
0.0001 |
1260 |
0.0002 |
TABLE 0400-45-01-.14(10)(h)
Reference (method or page number) |
||||||||||
Contaminant |
Methodology |
EPA1 |
EPA2 |
EPA3 |
EPA4 |
SM5 |
ASTM6 |
USGS7 |
DOE8 |
Other |
Naturally occurring:Gross alpha11and beta. |
Evaporation |
900.0 |
p 1 |
00-01 |
p 1 |
302, 7110 B |
R-1120-76 |
|||
Gross alpha11 |
Co-precipitation |
00-02 |
7110 C |
|||||||
Radium 226 |
Radon emanation |
903.1 |
p 16 |
Ra-04 |
p 19 |
7500-Ra C |
D 3454-91 |
R-1141-76 |
Ra-05 |
N.Y.9 |
Radio chemical |
903.0 |
p 13 |
Ra-03 |
304, 305, 7500-Ra B |
D 2460-91 |
R-1140-76 |
GA14 |
|||
Radium 228 |
Radio chemical |
904.0 |
p 24 |
Ra-05 |
p 19 |
304, 7500-Ra D |
R-1142-76 |
N. Y.9 N. J.10 |
||
Uranium12 |
Radio chemical |
908.0 |
7500-U B |
|||||||
Fluorometric |
908.1 |
7500-U C (17thEd.) |
D 2907-91 |
R-1180-76 R-1181-76 |
U-04 |
|||||
ICP-MS |
200.813 |
3125 |
D 5673-03 |
|||||||
Alpha spectrometry |
00-07 |
P 33 |
7500-U C (18th or 19thEd.) |
D 3972-90 |
R-1182-76 |
U-02 |
||||
Laser Phosophorimetry |
D 5174-91 |
|||||||||
Man-made Radioactive cesium. |
Radio chemical |
901.0 |
p 4 |
7500-Cs B |
D 2459-72 |
R-1111-76 |
||||
Gamma ray spectrometry. |
901.1 |
p 92 |
7120 (19thEd.) |
D 3649-91 |
R-1110-76 |
4.5.2.3 |
||||
Radioactive iodine |
Radio chemistry |
902.0 |
p 6 p 9 |
7500-I B 7500-I C 7500-I D |
D 3649-91 |
|||||
Gamma ray spectrometry. |
901.1 |
p 92 |
7120 (19thEd.) |
D 4785-88 |
4.5.2.3 |
|||||
Radioactive Strontium 89, 90. |
Radio chemical |
905.0 |
p 29 |
Sr-04 |
p 65 |
303, 7500-Sr B |
R-1160-76 |
Sr-01 Sr-02 |
||
Tritium |
Liquid scintillation |
906.0 |
p 34 |
H-02 |
p 87 |
306, 7500-3H B |
D 4107-91 |
R-1171-76 |
||
Gamma emitters |
Gamma ray |
901.1 |
p 92 |
7120 (19thEd.) |
D 3649-91 |
R-1110-76 |
4.5.2.3 |
|||
Spectrometry |
902.0 901.0 |
7500-Cs B 7500-I B |
D 4785-88 |
The procedures shall be done in accordance with the documents listed below. The incorporation by reference of documents 1 through 10 was approved by the Director of the Federal Register in accordance with 5 U.S.C. 552(a) and 1 CFR part 51. Copies of the documents may be obtained from the sources listed below. Information reguarding obtaining these documents can be obtained from the Safe Drinking Water Hotline at 800-426-4691. Documents may be inspected at EPA's Drinking Water Docket, 401 M Street, SW., Washington, DC 20460 (Telephone: 202-260-3027); or at the Office of Federal Register, 800 North Capitol Street, NW., Suite 700, Washington, DC.
Footnotes
1. "Prescribed Procedures for Measurement of Radioactivity in Drinking Water", EPA 600/4-80-032, August 1980. Available at U.S. Department of Commerce, National Technical Information Service (NTIS), 5285 Port Royal Road, Springfield, VA 22161 (Telephone 800-553-6847), PB 80-224744.
2. "Interim Radiochemical Methodology for Drinking Water", EPA 600/4-75-008(revised), March 1976. Available at NTIS, ibid. PB 253258.
3. "Radiochemistry Procedures Manual", EPA 520/5-84-006, December 1987. Available at NTIS, ibid. PB 84-215581.
4. "Radiochemical Analytical Procedures for Analysis of Environmental Samples", March 1979. Available at NTIS, ibid. EMSL LV 053917.
5. "Standard Methods for the Examination of Water and Wastewater", 13th, 17th, 18th, 19th Editions, 1971, 1989, 1992, 1995. Available at American Public Health Association, 1015 Fifteenth Street N. W., Washington, D.C. 20005. All methods are in the 17th, 18th, and 19th editions except 7500-U C Fluorometric Uranium was discontinued after the 17th Edition, 7120 Gamma Emitters is only in the 19th Edition, and 302, 303, 304, 305 and 306 are only in the 13th Edition.
6. Annual Book of ASTM Standards, Vol. 11.02, 1994. Available at American Society for Testing and Materials, 100 Barr Harbor Drive, West Conshohocken, PA 19428.
7. "Methods for Determination of Radioactive Substances in Water and Fluvial Sediments", Chapter A5 in Book 5 of Techniques of Water-Resources Investigations of the United States Geological Survey, 1977. Available at U. S. Geological Survey (USGS) Information Services, Box 25286, Federal Center, Denver, CO 80225-0425.
8. "EML Procedures Manual", 27th Edition, Volume 1, 1990. Available at the Environmental Measurements Laboratory, U.S. Department of Energy (DOE), 376 Hudson Street, New York, NY 10014-3621.
9. "Determination of Ra-226 and Ra-228 (Ra-02)", January 1980, Revised June 1982. Available at Radiological Sciences Institute Center for Laboratories and Research, New York State Department of Health, Empire State Plaza, Albany, NY 12201.
10. "Determination of Radium 228 in Drinking Water", August 1980. Available at State of New Jersey, Department of Environmental Protection, Division of Environmental Quality, Bureau of Radiation and Inorganic Analysis Services, 9 Ewing Street, Trenton, NJ 08625.
11. Natural uranium and thorium-230 are approved as gross alpha calibration standards for gross alpha with co-precipitation and evaporation methods; americium-241 is approved with co-precipitation methods.
12. If uranium (U) is determined by mass, a 0.67 pCi/ug of uranium conversion factor must be used. This conservative factor is based on the 1:1 activity ratio of U-234 to U-238 that is characteristic of naturally occurring uranium.
13. "Determination of Trace Elements in Waters and Wastes by Inductively Coupled Plasma-Mass Spectrometry," Revision 5.4, which is published in "Methods for the Determination of Metals in Environmental Samples-Supplement I," EPA 600-R-94-111, May 1994. Available at NTIS, PB 95-125472.
14. "The Determination of Radium-226 and Radium-228 in Drinking Water by Gamma-ray Spectrometry Using HPGE or Ge(Li) Detectors,'' Revision 1.2, December 2004. Available from the Environmental Resources Center, Georgia Institute of Technology, 620 Cherry Street, Atlanta, GA 30332-0335, USA, Telephone: 404-894-3776. This method may be used to analyze for radium-226 and radium-228 in samples collected after January 1, 2005 to satisfy the radium-226 and radium-228 monitoring requirements specified at 40 CFR 141.26.
TABLE 0400-45-01-.14(10)(h) 2
Contaminant |
Methodology |
SM 21 st Edition 1 |
ASTM 2 |
Naturally Occurring |
|||
Gross alpha and beta |
Evaporation |
7110 B |
|
Gross alpha |
Coprecipitation |
7110 C |
|
Radium 226 |
Radon emanation |
7500-Ra C |
D3454-05 |
Radiochemical |
7500-Ra B |
D2460-07 |
|
Radium 228 |
Radiochemical |
7500-Ra D |
|
Uranium |
Radiochemical |
7500-U B |
|
ICP-MS |
D5673-05 |
||
Alpha spectrometry |
7500-U C |
||
Laser Phosphorimetry |
D5174-07 |
||
Man-Made: |
|||
Radioactive Cesium |
Radiochemical |
7500-Cs B |
|
Gamma Ray Spectrometry |
7120 |
D3649-06 |
|
Radioactive Iodine |
Radiochemical |
7500-I B |
D3649-06 |
Gamma Ray Spectrometry |
7120 |
D4785-08 |
|
Radioactive Strontium 89, 90 |
Radiochemical |
7500-Sr B |
|
Tritium |
Liquid Scintillation |
7500-3H B |
D4107-08 |
Gamma Emitters |
Gamma Ray Spectrometry |
7120 7500-Cs B 7500-I B |
D3649-06 D4785-08 |
Footnotes
1 Standard Methods for the Examination of Water and Wastewater, 21st edition (2005). Available from American Public Health Association, 800 I Street NW, Washington, DC 20001- 3710
2 Available from ASTM International, 100 Barr Harbor Drive, West Conshohocken, PA 19428-2959 or http://astm.org. The methods listed are the only alternative versions that may be used.
TABLE 0400-45-01-.14(10)(i)
Methodology |
SM (19th or 20th ed) |
SM Online2 |
Other |
Residual measured1 |
||||
O3 |
Free Cl2 |
Combined Cl2 |
Total Cl2 |
ClO 2 |
||||
Amperometric Titration |
4500-Cl D |
4500-Cl-D-00 |
D 1253-033 |
X |
X |
X |
||
Low Level Amperometric Titration |
4500-Cl E |
4500-Cl-E-00 |
X |
|||||
DPD Ferrous Titrimetric |
4500-Cl F |
4500-Cl-F-00 |
X |
X |
X |
|||
DPD Colorimetric |
4500-Cl G |
4500-Cl-G-00 |
X |
X |
X |
|||
Syringaldazine (FACTS) |
4500-Cl H |
4500-Cl-H-00 |
X |
|||||
Iodometric Electrode |
4500-Cl I |
4500-Cl-I-00 |
X |
|||||
DPD |
4500-ClO2D |
X |
||||||
Amperometric Method II |
4500-ClO2E |
4500 ClO2 E-00 |
X |
|||||
Spectrophotometr ic |
327.0 Rev1.14 |
X |
||||||
Indigo Method |
4500-O3 B |
4500-O3 B-97 |
X |
Footnotes
1. X indicates method is approved for measuring specified disinfectant residual. Free chlorine or total chlorine may be measured for demonstrating compliance with the chlorine MRDL and combined chlorine, or total chlorine may be measured for demonstrating compliance with the chloramine MRDL.
2. The Standard Methods Online version that is approved is indicated by the last two digits in the method number which is the year of approval by the Standard Method Committee. Standard Methods Online are available at http://www.standardmethods.org.
3. Annual Book of ASTM Standards, Vol. 11.01, 2004 ; ASTM International; any year containing the cited version of the method may be used. Copies of this method may be obtained from ASTM International, 100 Barr Harbor Drive, P.O. Box C700 West Conshohocken, PA 19428-2959.
4. EPA Method 327.0, Revision 1.1, "Determination of Chlorine Dioxide and Chlorite Ion in Drinking Water Using Lissamine Green B and Horseradish Peroxidase with Detection by Visible Spectrophotometry,'' USEPA, May 2005, EPA 815-R-05-008. Available online at http://www.epa.gov/safewater/methods/sourcalt.html.
TABLE 0400-45-01-.14(10)(i) 1.
Residual |
Methodology |
SM 21 st Edition1 |
ASTM 2 |
Other |
Free Chlorine |
Amperometric Titration |
4500-Cl D |
D 1253-08 |
|
DPD Ferrous Titrimetric |
4500-Cl F |
|||
DPD Colorimetric |
4500-Cl G |
|||
Syringaldazine (FACTS) |
4500-Cl H |
|||
On-line Chlorine Analyzer |
EPA 334.0 3 |
|||
Amperometric Sensor |
Chlorosense 4 |
|||
Total Chlorine |
Amperometric Titration |
4500-Cl D |
D 1253-08 |
|
Amperometric Titration (Low level measurement) |
4500-Cl E |
|||
DPD Ferrous Titrimetric |
4500-Cl F |
|||
DPD Colorimetric |
4500-Cl G |
|||
Iodometric Electrode |
4500-Cl I |
|||
On-line Chlorine Analyzer |
EPA 334.0 3 |
|||
Amperometric Sensor |
Chlorosense 4 |
|||
Chlorine Dioxide |
Amperometric Titration |
4500-ClO2 C |
||
Amperometric Titration |
4500-ClO2 E |
|||
Ozone |
Indigo Method |
4500-O3 B |
Footnotes
1 Standard Methods for the Examination of Water and Wastewater, 21st edition (2005). Available from American Public Health Association, 800 I Street NW, Washington, DC 20001- 3710
2 Available from ASTM International, 100 Barr Harbor Drive, West Conshohocken, PA 19428-2959 or http://astm.org. The methods listed are the only alternative versions that may be used.
3 EPA Method 334.0. "Determination of Residual Chlorine in Drinking Water Using an On-line Chlorine Analyzer," September 2009. EPA 815-B-09-013. Available at http://epa.gov/safewater/methods/analyticalmethods_ogwdw.html.
4 Chlorosense. "Measurement of Free and Total Chlorine in Drinking Water by Palintest Chlorosense," August 2009. Available at http://www.nemi.gov or from Palintest Ltd, 21 Kenton Lands Road, PO Box 18395, Erlanger, KY 41018.
TABLE 0400-45-01-.14(10)(j)
Contaminant |
EPA |
ASTM3 |
SM418thand 19th ed. |
SM420th ed. |
SM7Online |
Other |
1. Aluminum |
200.72 200.82 200.92 |
3120 B 3113 B 3111 D |
3120 B |
3120 B-99 3113 B-99 3111 D-99 |
||
2. Chloride |
300.01 300.16 |
D4327-97, 03 D512-89 Reapproved 1999) B |
4110 B 4500-Cl - D 4500-Cl-B |
4110 B 4500-Cl - D 4500-Cl-B |
4110 B- 00 4500-Cl - D-97 4500-Cl-B-97 |
D6508, Rev. 28 |
3. Color |
2120 B |
2120 B |
2120 B-01 |
|||
4. Foaming Agents |
5540 C |
5540 C |
5540 C-00 |
|||
5. Iron |
200.72200.92 |
3120 B 3111 B 3113 B |
3120 B |
3120 B-99 3111 B-99 3113 B-99 |
||
6. Manganese |
200.72200.82200.92 |
3120 B 3111 B 3113 B |
3120 B |
3120 B-99 3111 B-99 3113 B-99 |
||
7. Odor |
2150 B |
2150 B |
2150 B-97 |
|||
8. Silver |
200.72 200.82 200.92 |
3120 B 3111 B 3113 B |
3120 B |
3120 B-99 3111 B-99 3113 B-99 |
I-3720-855 |
|
9. Sulfate |
300.01 300.16 375.21 |
D4327-97, 03 D516-90, 02 |
4110 B 4500-SO42-F 4500-SO42-C,D 4500-SO42-E |
4110 B 4500-SO42-F 4500-SO42-C,D 4500-SO42-E |
4110 B-00 |
D6508, Rev. 28 |
10. Total Dissolved Solids |
200.72 |
2540 C |
2540 C |
2540 C-97 |
||
11. Zinc |
200.82 |
3120 B 3111 B |
3120 B |
3120 B-99 3111 B-99 |
Footnotes
1. "Methods for the Determination of Inorganic Substances in Environmental Samples,'' EPA/600/R-93-100, August 1993. Available at NTIS, PB94-120821.
2. "Methods for the Determination of Metals in Environmental Samples-Supplement I,'' EPA/600/R-94-111, May 1994. Available at NTIS, PB 95-125472.
3. Annual Book of ASTM Standards, 1994, 1996, 1999, or 2004, Vols. 11.01 and 11.02, ASTM International; any year containing the cited version of the method may be used. Copies may be obtained from the ASTM International, 100 Barr Harbor Drive, West Conshohocken, PA 19428.
4. Standard Methods for the Examination of Water and Wastewater, 18th edition (1992), 19th edition (1995), or 20th edition (1998). American Public Health Association, 1015 Fifteenth Street, NW., Washington, DC 20005. The cited methods published in any of these three editions may be used, except that the versions of 3111 B, 3111 D, and 3113 B in the 20th edition may not be used.
5. Method I-3720-85, Techniques of Water Resources Investigation of the U.S. Geological Survey, Book 5, Chapter A-1, 3rd ed., 1989. Available from Information Services, U.S. Geological Survey, Federal Center, Box 25286, Denver, CO 80225-0425.
6. "Methods for the Determination of Organic and Inorganic Compounds in Drinking Water,'' Vol. 1, EPA 815-R-00-014, August 2000. Available at NTIS, PB2000-106981.
7. Standard Methods Online are available at http://www.standardmethods.org. The year in which each method was approved by the Standard Methods Committee is designated by the last two digits in the method number. The methods listed are the only online versions that may be used.
8. Method D6508, Rev. 2, "Test Method for Determination of Dissolved Inorganic Anions in Aqueous Matrices Using Capillary Ion Electrophoresis and Chromate Electrolyte,'' available from Waters Corp, 34 Maple St., Milford, MA, 01757, Telephone: 508/482-2131, Fax: 508/482-3625.
TABLE 0400-45-01-.14(10)(j) 1.
Contaminant |
Methodology |
EPA Method |
ASTM 1 |
SM 21 st Edition2 |
SM Online 3 |
Aluminum |
Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES) |
200.5, Revision 4.24 |
|||
Atomic Absorption; Direct |
3111 D |
||||
Atomic Absorption; Furnace |
3113 B |
||||
Inductively Coupled Plasma |
3120 B |
||||
Chloride |
Silver Nitrate Titration |
D 512-04 B |
4500-Cl - B |
||
Ion Chromatography |
4110 B |
||||
Potentiometric Titration |
4500-Cl - D |
||||
Color |
Visual Comparison |
2120 B |
|||
Foaming Agents |
Methylene Blue Active Substances (MBAS) |
5540 C |
|||
Iron |
Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES) |
200.5, Revision 4.2 |
|||
Atomic Absorption; Direct |
3111 B |
||||
Atomic Absorption; Furnace |
3113 B |
||||
Inductively Coupled Plasma |
3120 B |
||||
Manganese |
Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES) |
200.5, Revision 4.2 |
|||
Atomic Absorption; Direct |
3111 B |
||||
Atomic Absorption; Furnace |
3113 B |
||||
Inductively Coupled Plasma |
3120 B |
||||
Odor |
Threshold Odor Test |
2150 B |
|||
Silver |
Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES) |
200.5, Revision 4.2 |
|||
Atomic Absorption; Direct |
3111 B |
||||
Atomic Absorption; Furnace |
3113 B |
||||
Inductively |
3120 B |
||||
Coupled Plasma |
|||||
Sulfate |
Ion Chromatography |
4110 B |
|||
Gravimetric with ignition of residue |
4500-SO4 -2 C |
4500-SO4 -2 C-97 |
|||
Gravimetric with drying of residue |
4500-SO4 -2 D |
4500-SO4 -2 D-97 |
|||
Turbidimetric method |
4500-SO4 -2 E |
4500-SO4 -2 E-97 |
|||
Automated methylthymol blue method |
4500-SO4 -2 F |
4500-SO4 -2 F-97 |
|||
Total Dissolved Solids |
Total Dissolved Solids Dried at 180 deg C |
2540 C |
|||
Zinc |
Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES) |
200.5, Revision 4.2 |
|||
Atomic Absorption; Direct Aspiration |
3111 B |
||||
Inductively Coupled Plasma |
3120 B |
Footnotes
1 Available from ASTM International, 100 Barr Harbor Drive, West Conshohocken, PA 19428-2959 or http://astm.org. The methods listed are the only alternative versions that may be used.
2 Standard Methods for the Examination of Water and Wastewater, 21st edition (2005). Available from American Public Health Association, 800 I Street NW, Washington, DC 20001- 3710
3 Standard Methods Online are available at http://www.standardmethods.org. The year in which each method was approved by the Standard Methods Committee is designated by the last two digits in the method number. The methods listed are the only online versions that may be used.
4 EPA Method 200.5, Revision 4.2. "Determination of Trace Elements in Drinking Water by Axially Viewed Inductively Coupled Plasma-Atomic Emission Spectrometry." 2003. EPA/600/R-06/115. (Available at http://www.epa.gov/nerlcwww/ordmeth.htm.)
TABLE 0400-45-01-.14(10)(k) 1.
Approved Methods for Disinfection Byproduct Compliance Monitoring
Contaminant and methodology1 |
EPA method |
Standard method2 |
SM online9 |
ASTM method3 |
TTHM |
||||
P&T/GC/ElCD & PID |
502.24 |
|||
P&T/GC/MS |
524.2 |
|||
LLE/GC/ECD |
551.1 |
|||
HAA5 |
||||
LLE (diazomethane)/GC/ECD |
6251 B5 |
6251 B-94 |
||
SPE (acidic methanol)/GC/ECD |
552.15 |
|||
LLE (acidic methanol)/GC/ECD |
552.2, 552.3 |
|||
Bromate |
||||
Ion chromatography |
300.1 |
D 6581-00 |
||
Ion chromatography & post column reaction |
317.0 Rev 2.06, 326.06 |
|||
IC/ICP-MS |
321.86,7 |
|||
Chlorite |
||||
Amperometric titration |
4500-ClO2 E8 |
4500-ClO2 E-00 8 |
||
Spectrophotometry |
327.0 Rev 1.18 |
|||
Ion chromatography |
300.0, 300.1, 317.0 Rev 2.0, 326.0. |
D 6581-00 |
1P&T=purge and trap; GC=gas chromatography; ElCD=electrolytic conductivity detector; PID=photoionization detector; MS=mass spectrometer; LLE=liquid/liquid extraction; ECD=electron capture detector; SPE=solid phase extraction; IC=ion chromatography; ICP-MS=inductively coupled plasma/mass spectrometer.
2 19 th and 20th editions of Standard Methods for the Examination of Water and Wastewater, 1995 and 1998, respectively, American Public Health Association; either of these editions may be used.
3 Annual Book of ASTM Standards, 2001 or any year containing the cited version of the method, Vol 11.01.
4If TTHMs are the only analytes being measured in the sample, then a PID is not required.
5The samples must be extracted within 14 days of sample collection.
6Ion chromatography & post column reaction or IC/ICP-MS must be used for monitoring of bromate for purposes of demonstrating eligibility of reduced monitoring, as prescribed in Rule 0400-45-01-.36(6)(b) 3.(ii).
7Samples must be preserved at the time of sampling with 50 mg ethylenediamine (EDA)/L of sample and must be analyzed within 28 days.
8Amperometric titration or spectrophotometry may be used for routine daily monitoring of chlorite at the entrance to the distribution system, as prescribed in Rule 0400-45-01-.36(6)(b) 2.(i)(I). Ion chromatography must be used for routine monthly monitoring of chlorite and additional monitoring of chlorite in the distribution system, as prescribed in Rules 0400-45-01-.36(6)(b) 2.(i)(II) and (b)2.(ii).
9The Standard Methods Online version that is approved is indicated by the last two digits in the method number which is the year of approval by the Standard Method Committee. Standard Methods Online are available at http://www.standardmethods.org.
TABLE 0400-45-01-.14(10)(k) 1.(i)
Contaminant |
Methodology |
EPA Method |
ASTM 1 |
SM 21 st Edition2 |
TTHM |
P&T/GC/MS |
524.3 3 |
||
HAA5 |
LLE (diazomethane)/GC/ECD |
6251 B |
||
Ion Chromatography Electrospray Ionization Tandem Mass Spectrometry (IC-ESI-MS/MS) |
557 4 |
|||
Bromate |
Two-Dimensional Ion Chromatography (IC)* |
302.0 5 |
||
Ion Chromatography Electrospray Ionization Tandem Mass Spectrometry (IC-ESI-MS/MS)* |
557 4 |
|||
Chemically Supressed Ion Chromatography |
D 6581-08 A |
|||
Electrolytically Supressed Ion Chromatography |
D 6581-08 B |
|||
Chlorite |
Chemically Supressed Ion Chromatography |
D 6581-08 A |
||
Electrolytically Supressed Ion Chromatography |
D 6581-08 B |
|||
Chlorite - daily |
Amperometric Titration |
4500-ClO2 E |
||
monitoring as prescribed in Rule 0400-45-01-.36(6)(b) 2.(i)(I) |
Footnotes
1 Available from ASTM International, 100 Barr Harbor Drive, West Conshohocken, PA 19428-2959 or http://astm.org. The methods listed are the only alternative versions that may be used.
2 Standard Methods for the Examination of Water and Wastewater, 21st edition (2005). Available from American Public Health Association, 800 I Street NW, Washington, DC 20001- 3710
3 EPA Method 524.3, Version 1.0. "Measurement of Purgeable Organic Compounds in Water by Capillary Column Gas Chromatography/Mass Spectrometry," June 2009. EPA 815-B-09-009. Available at http://epa.gov/safewater/methods/analyticalmethods_ogwdw.html.
4 EPA Method 557. "Determination of Haloacetic Acids, Bromate, and Dalapon in Drinking Water by Ion Chromatography Electrospray Ionization Tandem Mass Spectrometry (IC-ESI-MS/MS)," September 2009. EPA 815-B-09-012. Available at http://epa.gov/safewater/methods/analyticalmethods_ogwdw.html.
5 EPA Method 302.0. "Determination of Bromate in Drinking Water using Two-Dimensional Ion Chromatography with Supressed Conductivity Detection," September 2009. EPA 815-B-09-014. Available at http://epa.gov/safewater/methods/analyticalmethods_ogwdw.html.
*Only IC and IC-ESI-MS/MS have an MRL <0.0010 mg/l and may be used for Bromate monitoring listed under EPA 815-B-09-014. Available at http://epa.gov/safewater/methods/analyticalmethods_ogwdw.html.
DBP |
Acceptance limits (percent of true value) |
Comments |
TTHM |
||
Chloroform |
±20 |
Laboratory must meet all 4 individual THM acceptance limits in order to successfully pass a PE sample for TTHM |
Bromodichloromethane |
±20 |
|
Dibromochloromethane |
±20 |
|
Bromoform |
±20 |
|
HAA5 |
||
Monochloroacetic Acid |
±40 |
Laboratory must meet the acceptance limits for all 5 of the HAA5 compounds in order to successfully pass a PE sample for HAA5 |
Dichloroacetic Acid |
±40 |
|
Trichloroacetic Acid |
±40 |
|
Monobromoacetic Acid |
±40 |
|
Dibromoacetic Acid |
±40 |
|
Chlorite |
±30 |
|
Bromate |
±30 |
DBP |
Minimum reporting level (mg/L)1 |
Comments |
TTHM2 |
||
Chloroform |
0.0010 |
|
Bromodichloromethane |
0.0010 |
|
Dibromochloromethane |
0.0010 |
|
Bromoform |
0.0010 |
|
HAA52 |
||
Monochloroacetic Acid |
0.0020 |
|
Dichloroacetic Acid |
0.0010 |
|
Trichloroacetic Acid |
0.0010 |
|
Monobromoacetic Acid |
0.0010 |
|
Dibromoacetic Acid |
0.0010 |
|
Chlorite |
0.020 |
Applicable to monitoring as prescribed in Rules 0400-45-01-.36(6)(b) 2(i)(II) and (b)2(ii). |
Bromate |
0.0050 or 0.00010 |
Laboratories that use EPA Methods 317.0 Revision 2.0, 326.0 or 321.8 must meet a 0.0010 mg/L MRL for bromate. The alternative methods for reduced monitoring under Rule 0400-45-01-.36(6)(b) 3.(ii)(II) meeting an MRL of < 0.0010 mg/L are EPA Method 302.0 and 557. |
1 The calibration curve must encompass the regulatory minimum reporting level (MRL) concentration. Data may be reported for concentrations lower than the regulatory MRL as long as the precision and accuracy criteria are met by analyzing an MRL check standard at the lowest reporting limit chosen by the laboratory. The laboratory must verify the accuracy of the calibration curve at the MRL concentration by analyzing an MRL check standard with a concentration less than or equal to 110% of the MRL with each batch of samples. The measured concentration for the MRL check standard must be ±50% of the expected value, if any field sample in the batch has a concentration less than 5 times the regulatory MRL. Method requirements to analyze higher concentration check standards and meet tighter acceptance criteria for them must be met in addition to the MRL check standard requirement.
2 When adding the individual trihalomethane or haloacetic acid concentrations to calculate the TTHM or HAA5 concentrations, respectively, a zero is used for any analytical result that is less than the MRL concentration for that DBP, unless otherwise specified by the Department.
http://www.archives.gov/federal_register/code_of_federal_regulations/ibr_locations.html.
Notes
Authority: T.C.A. §§ 4-5-201, et seq.; 68-203-101, et seq.; and 68-221-701, et seq.
State regulations are updated quarterly; we currently have two versions available. Below is a comparison between our most recent version and the prior quarterly release. More comparison features will be added as we have more versions to compare.