Tenn. Comp. R. & Regs. 0400-45-01-.41 - REVISED TOTAL COLIFORM RULE
Organism |
Methodology category |
Method1 |
Citation1 |
Total Coliforms |
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Lactose Fermentation Methods |
Standard Total Coliform Fermentation Technique |
Standard Methods 9221 B.1, B.2 (20thed.; 21sted.)23Standard Methods Online 9221 B.1, B.2-9923 |
|
Presence-Absence (PA) Coliform Test |
Standard Methods 9221 D.1, D.2 (20thed.; 21sted.)27Standard Methods Online 9221 D.1, D.2-9927 |
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|
Standard Total Coliform Membrane Filter Procedure |
Standard Methods 9222 B, C (20thed.; 21sted.)24 Standard Methods Online 9222 B-9724,9222 C-9724 |
|
4 Chromocult®24 |
|
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Enzyme Substrate Methods |
Colilert® |
Standard Methods 9223 B (20thed.; 21sted.)25 Standard MethodsOnline 9223 B-9725 |
|
Colisure® |
Standard Methods 9223 B (20thed.; 21sted.)256 Standard MethodsOnline 9223 B-97256 |
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E*Colite® Test2Readycult® Test2 |
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Modified Colitag® Test2 |
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Escherichia coli. |
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Escherichia coli Procedure (following Lactose Fermentation Methods). |
EC-MUG medium |
Standard Methods 9221 F.1 (20thed.; 21sted.)2 |
|
Escherichia coli Partition Method |
EC broth with MUG (EC-MUG) |
Standard Methods 9222 G.1c(2) (20thed.; 21sted.)28 |
|
NA-MUG medium |
Standard Methods 9222 G.1c(1) (20thed.; 21sted.)2 |
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|
4 Chromocult®24 |
|
|
Enzyme Substrate Methods |
Colilert® |
Standard Methods 9223 B (20thed.; 21sted.)25 Standard Methods Online 9223 B-97256 |
|
Colisure® |
Standard Methods 9223 B (20th ed.; 21st ed.)256Standard MethodsOnline 9223 B-97256 |
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E*Colite® Test2Readycult® Test2Modified Colitag®Test2 |
1 The procedures must be done in accordance
with the documents listed in footnote 2 below, incorporation by reference. For
Standard Methods, either editions, 20th (1998) or 21st (2005), may be used. For
the Standard Methods Online, the year in which each method was approved by the
Standard Methods Committee is designated by the last two digits following the
hyphen in the method number. The methods listed are the only online versions
that may be used. For vendor methods, the date of the method listed in footnote
2 is the date/version of the approved method. The methods listed are the only
versions that may be used for compliance with this rule. Laboratories should be
careful to use only the approved versions of the methods, as product
2 Incorporation by reference:
3 Lactose broth,
as commercially
4 All
5 Multiple-tube and multi-well enumerative formats for this method are approved for use in presence-absence determination under this rule.
6 Colisure® results may be read after an incubation time of 24 hours.
7 A multiple tube enumerative format, as described in Standard Methods for the Examination of Water and Wastewater 9221, is approved for this method for use in presence-absence determination under this regulation.
8 The following changes must be made to the EC broth with MUG (EC-MUG) formulation: Potassium dihydrogen phosphate, KH2PO4, must be 1.5g, and 4-methylumbelliferyl-Beta-D-glucuronide must be 0.05 g.
Total Coliform Monitoring Frequency for Public Water Systems Serving More than 1,000 People
Population Served |
Minimum number of samples per month |
1,001 to 2,500 |
2 |
2,501 to 3,300 |
3 |
3,301 to 4,100 |
4 |
4,101 to 4,900 |
5 |
4,901 to 5,800 |
6 |
5,801 to 6,700 |
7 |
6,701 to 7,600 |
8 |
7,601 to 8,500 |
9 |
8,501 to 12,900 |
10 |
12,901 to 17,200 |
15 |
17,201 to 21,500 |
20 |
21,501 to 25,000 |
25 |
25,001 to 33,000 |
30 |
33,001 to 41,000 |
40 |
41,001 to 50,000 |
50 |
50,001 to 59,000 |
60 |
59,001 to 70,000 |
70 |
70,001 to 83,000 |
80 |
83,001 to 96,000 |
90 |
96,001 to 130,000 |
100 |
130,001 to 220,000 |
120 |
220,001 to 320,000 |
150 |
320,001 to 450,000 |
180 |
450,001 to 600,000 |
210 |
600,001 to 780,000 |
240 |
780,001 to 970,000 |
270 |
970,001 to 1,230,000 |
300 |
1,230,001 to 1,520,000 |
330 |
1,520,001 to 1,850,000 |
360 |
1,850,001 to 2,270,000 |
390 |
2,270,001 to 3,020,000 |
420 |
3,020,001 to 3,960,000 |
450 |
3,960,000 or more |
480 |
Notes
State regulations are updated quarterly; we currently have two versions available. Below is a comparison between our most recent version and the prior quarterly release. More comparison features will be added as we have more versions to compare.