(A)
Until one year after the effective date of this
rule, microcystins analysis shall be performed by a laboratory found to be
acceptable to the Ohio environmental protection agency. Beginning one year from
the effective date of this rule, microcystins
Microcystins analysis shall be performed by a
laboratory certified by the director pursuant to Chapter 3745-89 of the
Administrative Code. However, if a laboratory found to be acceptable submits a
complete application for certification in accordance with rule
3745-89-04 of the Administrative
Code, the laboratory may continue to perform microcystins analysis until action
is taken on the application.
(B)
Until the director determines there is sufficient
certified laboratory capacity for cyanobacteria screening and no earlier than
one year after the effective date of this rule, the Ohio environmental
protection agency division of environmental services will perform cyanobacteria
screening. After the director determines there is sufficient certified
laboratory capacity for cyanobacteria screening, cyanobacteria
Cyanobacteria screening required by this chapter shall
be performed by a laboratory certified by the director pursuant to Chapter
3745-89 of the Administrative Code.
(C) In addition, analysis required by this
chapter shall be conducted in accordance with the following analytical methods
and time frames:
(1) Cyanobacteria screening:
"Ohio EPA DES method 705.0, Ohio EPA quantitative
Polymerase Chain Reaction (qPCR) Multi-Plex
Molecular Assay for Determination of Cyanobacteria and Cyanotoxin-Producing
Genes Analytical Methodology" version 1.0 (September 2018)
method accepted by the director or another
method accepted by the director in writing. Samples must be analyzed within
seven days of collection.
(2)
Microcystins: "Ohio EPA DES method 701.0, Ohio EPA Total (Extracellular and
Intracellular) Microcystins - ADDA by ELISA Analytical Methodology" version
2.2 (November 2015)
2.4 (November 2021) or another method accepted by the
director in writing. Except where otherwise noted in this chapter and
notwithstanding the holding time specified in the method, samples must be
analyzed within five days of collection.
(D) Reporting of analytical data for
determining compliance with this chapter shall be completed in accordance with
rule
3745-89-08 of the Administrative
Code.
(E)
Failure to meet paragraph (D) of this rule is a
reporting violation and requires the public water system to provide tier 3
public notification in accordance with rule 3745-81-32 of the Administrative
Code.
[Comment: This rule incorporates the "Ohio EPA DES method
701.0, Ohio EPA Total (Extracellular and Intracellular) Microcystins - ADDA by
ELISA Analytical Methodology" version 2.2 (November
2015)
2.4 (November 2021) and "Ohio EPA DES
method 705.0, Ohio EPA quantitative Polymerase Chain Reaction (qPCR) Multi-Plex
Molecular Assay for Determination of Cyanobacteria and CyanotoxinProducing
Genes Analytical Methodology" version 1.0 (September 2018) by reference.
Copies are available at www.epa.ohio.gov/ddagw/HAB.aspx
and from the "Ohio EPA Lazarus Government Center, 50 West Town Street, Suite
700, Columbus, OH 43215." Copies can also be obtained by contacting the
laboratory certification office at (614) 644-4245.]
Notes
Ohio Admin. Code
3745-90-04
Effective:
11/1/2022
Five Year Review (FYR) Dates:
3/30/2022 and
11/01/2027
Promulgated
Under: 119.03
Statutory
Authority: 3745.50,
6109.04
Rule
Amplifies: 3745.50,
6109.04
Prior
Effective Dates: 06/01/2016
