(A)
Until one year
after the effective date of this rule, microcystins analysis shall be performed
by a laboratory found to be acceptable to the Ohio environmental protection
agency. Beginning one year from the effective date of this rule,
microcystins
Microcystins analysis shall
be performed by a laboratory certified by the director pursuant to Chapter
3745-89 of the Administrative Code. However, if a laboratory found to be
acceptable submits a complete application for certification in accordance with
rule
3745-89-04
of the Administrative Code, the laboratory may continue to perform microcystins
analysis until action is taken on the application.
(B)
Until the
director determines there is sufficient certified laboratory capacity for
cyanobacteria screening and no earlier than one year after the effective date
of this rule, the Ohio environmental protection agency division of
environmental services will perform cyanobacteria screening. After the director
determines there is sufficient certified laboratory capacity for cyanobacteria
screening, cyanobacteria
Cyanobacteria
screening required by this chapter shall be performed by a laboratory certified
by the director pursuant to Chapter 3745-89 of the Administrative
Code.
(C) In addition, analysis
required by this chapter shall be conducted in accordance with the following
analytical methods and time frames:
(1)
Cyanobacteria screening:
"Ohio EPA DES method 705.0,
Ohio EPA quantitative Polymerase Chain Reaction (qPCR
) Multi-Plex Molecular Assay for Determination of
Cyanobacteria and Cyanotoxin-Producing Genes Analytical Methodology" version
1.0 (September 2018)
method accepted by the
director or another method accepted by the director in writing.
Samples must be analyzed within seven days of collection.
(2) Microcystins: "Ohio EPA DES method 701.0,
Ohio EPA Total (Extracellular and Intracellular) Microcystins - ADDA by ELISA
Analytical Methodology" version
2.2 (November
2015)
2.4 (November 2021) or another
method accepted by the director in writing. Except where otherwise noted in
this chapter and notwithstanding the holding time specified in the method,
samples must be analyzed within five days of collection.
(D) Reporting of analytical data for
determining compliance with this chapter shall be completed in accordance with
rule
3745-89-08
of the Administrative Code.
(E)
Failure to meet paragraph (D) of this rule is a
reporting violation and requires the public water system to provide tier 3
public notification in accordance with rule
3745-81-32 of
the Administrative Code.
[Comment: This rule incorporates the "Ohio EPA DES method
701.0, Ohio EPA Total (Extracellular and Intracellular) Microcystins - ADDA by
ELISA Analytical Methodology" version 2.2 (November
2015)
2.4 (November 2021) and "Ohio EPA DES
method 705.0, Ohio EPA quantitative Polymerase Chain Reaction (qPCR) Multi-Plex
Molecular Assay for Determination of Cyanobacteria and CyanotoxinProducing
Genes Analytical Methodology" version 1.0 (September 2018) by reference.
Copies are available at www.epa.ohio.gov/ddagw/HAB.aspx
and from the "Ohio EPA Lazarus Government Center, 50 West Town Street, Suite
700, Columbus, OH 43215." Copies can also be obtained by contacting the
laboratory certification office at (614) 644-4245.]
Notes
Ohio Admin. Code 3745-90-04
Effective:
11/1/2022
Five Year Review (FYR) Dates:
3/30/2022 and
11/01/2027
Promulgated
Under:
119.03
Statutory Authority:
3745.50,
6109.04
Rule Amplifies:
3745.50,
6109.04
Prior Effective Dates:
06/01/2016
