Iowa Admin. Code r. 567-41.7 - Groundwater rule: sanitary survey, microbial source water monitoring, treatment technique
* The
* The system only has one groundwater source required to be sampled,
* The system has no treatment, and
* Should the source water sample beE. coli
-positive, the system would incur an acute coliform bacteria
* A wholesale groundwater system that receives notice from a consecutive system it serves that a sample collected under 41.2(1)"f" through 41.2(1)"i" is total coliform-positive must, within 24 hours of being notified, collect triggered sample(s) from its groundwater source(s) under 41.7(3)"a" (2) and analyze the sample(s) for a fecal indicator.
* If the triggered source sample(s) is fecal indicator-positive, the wholesale groundwater system must notify all consecutive systems served by that groundwater source of the fecal indicator-positive result within 24 hours of being notified of the result and must collect the required additional five samples from the source within 24 hours under 41.7(3)"a" (3).
Analytical Methods for Source Water Monitoring
|
Fecal Indicator1 |
Methodology |
Method Citation |
|
E. coli |
Colilert3 |
9223B2,12,13 9223 B-97, B-0418 |
|
Coli sure3 |
9223B2,12,13 9223B-97, B-0418 |
|
|
Membrane filter method with MI agar |
EPA Method 16044 |
|
|
Colilert-18 |
9223B2,12,13 9223B-97, B-0418 |
|
|
m-ColiBlue24 Test5 |
||
|
E*Colite Test6 |
||
|
EC-MUG7 |
9221F2,13 9221 F-0618 |
|
|
NA-MUG7 |
9222G2 |
|
|
Readycult |
Readycult14 |
|
|
Colitag |
Modified Colitag15 |
|
|
Chromocult |
Chromocult16 |
|
|
Tecta EC/TC |
Tecta EC/TC 19 |
|
|
Enterococci |
Multiple-tube technique |
9230B29230 B-0418 |
|
Membrane filter technique |
9230C2 |
|
|
Membrane filter technique |
EPA Method 16008 |
|
|
Enterolert9 |
||
|
Coliphage |
Two-step enrichment presence-absence procedure |
EPA Method 160110, FastPhage17 |
|
Single agar layer procedure |
EPA Method 160211 |
Analyses must be conducted in accordance with the documents
listed below. The
The address for EPA's Water Resource Center, referenced in several of the footnotes, is EPA Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW, Washington, DC 20460.
1The time from sample collection to initiation of analysis may not exceed 30 hours. The groundwater system is encouraged but is not required to hold samples below 10°C during transit.
2Methods are described in
3Medium is available through IDEXX Laboratories, Inc., One IDEXX Drive, Westbrook, ME 04092.
4EPA Method 1604: Total
Coliforms andEscherichia coli in Water by
5A description of the
m-ColiBlue24 Test, "Total Coliforms andE. coli
6A description of the E*Colite Test, "Charm E*Colite Presence/Absence Test for Detection and Identification of Coliform Bacteria andEscherichia coli in Drinking Water," January 9, 1998, is available from Charm Sciences, Inc., 659 Andover Street, Lawrence, MA 01843-1032.
7EC-MUG (Method 922 IF) or NA-MUG (Method 9222G) can be used for E. coli testing step as described in 41.2(1)"f"(6) or (7) after use of Standard Method 9221B, 9221D, 9222B, or 9222C.
8EPA Method 1600: Enterococci in
Water by
9Medium is available through IDEXX Laboratories, Inc., One IDEXX Drive, Westbrook, ME 04092. Preparation and use of the medium is set forth in the article "Evaluation of Enterolert for Enumeration of Enterococci in Recreational Waters" by Budnick, G.E., Howard, R.T., and Mayo, D.R., 1996, Applied and Environmental Microbiology, 62:3881-3884.
10EPA Method 1601: Male-Specific (F+) and Somatic Coliphage in Water by Two-Step Enrichment Procedure; April 2001, EPA 821-R-01-030. Method is available at www.nemi.gov.
"EPA Method 1602: Male-Specific (F+) and Somatic Coliphage in Water by Single Agar Layer (SAL) Procedure; April 2001, EPA 821-R-01-029. Method is available at www.nemi.gov.
12
13
14Readycult Method, "Readycult Coliforms 100 Presence/Absence Test for Detection and Identification of Coliform Bacteria andEscherichia coli in Finished Waters," January 2007, Version 1.1. Available from EMD Millipore, 290 Concord Road, Billerica, MA 01821.
15Modified Colitag Method, "Modified Colitag Test Method for the Simultaneous Detection of E.coli and Other Total Coliforms in Water (ATP D05-0035)," August 28, 2009. Available from www.nemi.gov or CPI International, 5580 Skylane Blvd., Santa Rosa, CA 95403.
16Chromocult Method, "Chromocult Coliform Agar Presence/Absence Membrane Filter Test Method for Detection and Identification of Coliform Bacteria andEscherichia coli in Finished Waters," November 2000, Version 1.0. Available from EMD Millipore, 290 Concord Road, Billerica, MA 01821.
17Charm Sciences, Inc., "FastPhage Test Procedure. Presence/Absence for Coliphage in Ground Water with Same Day Positive Prediction," Version 009, November 2012. Available atwww.charmsciences.com.
18
19TectaEC/TC. "Presence/Absence Method for Simultaneous Detection of Total Coliforms andEscherichia coli in Drinking Water," April 2014. Available from Veolia Water Solutions and Technologies, Suite 4697, Biosciences Complex, 116 Barrie Street, Kingston, Ontario, Canada K7L 3N6.
* Any subsequent modifications to a
* If the
* The nature of the
* The
* For systems with a large proportion of non-English
speaking consumers, as determined by the
* A groundwater system serving more than 3,300 people must
continuously monitor the
* A groundwater system serving 3,300 or fewer people must
monitor the
* The membrane has an absolute molecular weight cut-off (MWCO), or an alternate parameter that describes the exclusion characteristics of the membrane, that can reliably achieve at least 4-log removal of viruses;
* The membrane
* The integrity of the membrane is intact.
* Monitor the alternative treatment in accordance with all
* Operate the alternative treatment in accordance with all
compliance requirements that the
Notes
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