Utah Admin. Code R58-21-3 - Trichomoniasis - Sampling and Testing Procedures
(1) Sample collection - Samples are obtained
by a certified veterinarian via a vigorous scraping of the bull's prepuce using
a sterile syringe and new pipette on each bull.
(2) Sample handling - Samples shall be
transferred and transported in an approved transfer tube . Media should be
maintained at 65 to 90 degrees Fahrenheit, 18 to 32 degrees Celsius, during
sampling and transport to clinic. Samples shall be set up for incubation within
24 hours of sampling. Samples shall also be protected from direct
sunlight.
(3) Polymerase Chain
Reaction (PCR) testing - The inoculated media shall be incubated at 98 degrees
Fahrenheit, 37 degrees Celsius, for 24 hours and then frozen. Samples may
remain frozen for up to 3 weeks. Samples from herds under investigation or
quarantine shall be mailed within 72 hours. The frozen sample shall be sent
overnight on postal approved frozen packs to the Utah Veterinary Diagnostic
Laboratory at 950 East 1400 North, Logan, Utah 84341 or a laboratory approved
by the State Veterinarian for PCR testing.
(4) Pool samples- At the State Veterinarian's
discretion, herds at high risk or under investigation for Trichomoniasis may be
required to have individual bull tests.
(5) Test results shall be recorded on test
charts provided by the department.
(6) Test charts shall have the following
information:
(a) veterinarian's name and
contact information;
(b) owner's
name and contact information;
(c)
bull's trichomoniasis tag number, RFID tag number, age, and breed;
(d) the date of collection; and
(e) the finalized test results.
(7) A copy of each test chart
shall be submitted to the department within seven days of receiving the
results.
Notes
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